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pDONR223-MET

Plasmid

#23889

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MET (MET Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-IDH2-FLAG

Plasmid

#66806

Purpose

Tet-inducible expression of IDH2 in mammalian cells

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH2 (IDH2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ssSBP-EGFP

Plasmid

#65277

Purpose

to synchronize transport of secretory soluble GFP from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

EGFP

Tags

IL-2 signal sequence and Streptavidin Binding Pr…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP

Plasmid

#111584

Purpose

An AAV vector that expresses myristoylated mScarlet-I and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrmScarlet-I-P2A-post-eGRASP

Use

AAV

Promoter

tetO

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1[85Q]

Plasmid

#33237

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Tags

Flag

Expression

Mammalian

Mutation

insert contains a stretch of 85 Glutamines and no…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5-H2B-AID-EYFP

Plasmid

#47329

Purpose

for plasmid integration using the Flp-In system. AID at C-terminal

Depositor

Don Cleveland

Article

Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

Insert

histone H2B (H2BC21 Human)

Tags

AID and EYFP

Expression

Mammalian

Available Since

Aug. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pRK7

Plasmid

#10883

Depositor

John Blenis

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pWZL Blast DN E-cadherin

Plasmid

#18800

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

Dom neg E-cadherin (Cdh1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

truncated form of E-cadherin (dominant negative) …

Available Since

July 15, 2008

Availability

Academic Institutions and Nonprofits only

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pMXs-mRFP1

Plasmid

#21315

Purpose

MoMLV-based Retroviral vector, expresses mRFP1

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

monomeric Red Fluorescence Protein 1

Use

Retroviral

Expression

Mammalian

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST PUM2

Plasmid

#40292

Depositor

Thomas Tuschl

Article

Hafner et al Cell. 2010 Apr 2;141(1):129-41.

Insert

Pumilio homolog 2 (PUM2 Human)

Tags

FLAG-HA

Expression

Mammalian

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

Plasmid

#20943

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

H134R

Promoter

CaMKIIa

Available Since

July 9, 2009

Availability

Academic Institutions and Nonprofits only

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pUV15tetORm

Plasmid

#17975

Depositor

Sabine Ehrt

Article

Guo et al J Bacteriol. 2007 Jul . 189(13):4614-23.

Insert

pmyc1tetO-gfp, tetR(B)

Use

Mycobacteria expression

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pMCP-Luc (MCP-1 promoter in pGL3-basic)

Plasmid

#40324

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

MCP-1 promoter (Ccl2 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

contains MCP-1 promoter region through the nucleo…

Promoter

MCP-1

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pBT378_pattB-pCA-GFP-pA-attB

Plasmid

#52554

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5 TBRI-His

Plasmid

#19161

Depositor

Joan Massague

Insert

transforming growth factor, beta receptor 1 (TGFBR1 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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PB-TET

Plasmid

#20909

Purpose

piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Gateway Destination Vector

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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MIGR-mFoxP3

Plasmid

#24067

Depositor

Dan Littman

Insert

Foxp3 (Foxp3 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pDIRE

Plasmid

#26745

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

iCre expression cassette

Use

Cre/Lox; Flp/frt

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pPGK-Cre-bpA

Plasmid

#11543

Purpose

Expresses Cre recombinase from mouse PGK promoter in mammalian cells.

Depositor

Klaus Rajewsky

Insert

Phosphoglycerate kinase 1 promoter

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_DESTFLAGHA

Plasmid

#26361

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Type

Empty backbone

Tags

FLAG/HA

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only