59,544 results

1. TFORF0098

   Plasmid

   #142460

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MSGN1 (MSGN1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1245 pCMVb p300

   Plasmid

   #10717

   Depositor

   William Sellers

   Insert

   p300 (EP300 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-PIK3R5

   Plasmid

   #23819

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PIK3R5 (PIK3R5 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. P2RY6-Tango

   Plasmid

   #66476

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   P2RY6 (P2RY6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. betaTrCP mVenus reporter

   Plasmid

   #192458

   Purpose

   Fluorescent reporter for beta-TrCP activity in human cells

   Depositor

   Steven Cappell

   Article

   Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

   Insert

   CDC25B (CDC25B Human)
   

   Use

   Lentiviral

   Tags

   SV40 NLS and mVenus

   Expression

   Mammalian

   Mutation

   aa198-338

   Promoter

   EF1alpha

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. betaTrCP mVenus mutant reporter

   Plasmid

   #192459

   Purpose

   Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

   Depositor

   Steven Cappell

   Article

   Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

   Insert

   CdC25B
   

   Use

   Lentiviral

   Tags

   SV40 NLS and mVenus

   Expression

   Mammalian

   Mutation

   aa198-338 of CDC25B. betaTrCP degron sequence (aa…

   Promoter

   EF1alpha

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV uN2CpolyGly op GFP (NIID)

   Plasmid

   #224356

   Purpose

   Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)

   Depositor

   Nicolas Charlet-Berguerand

   Article

   Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

   Insert

   uN2C with a GGC repeat expansion (100x)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Codon-optimized for expression in human, so no pu…

   Promoter

   CMV + chimeric intron (CAG)

   Available Since

   Feb. 5, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pGTag-gal4-VP16-B-actin

   Plasmid

   #117817

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   gal4/VP16
   

   Use

   Synthetic Biology

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBJ FLAG-hPar1

   Plasmid

   #53226

   Purpose

   mammalian expression of human Par1 with N-terminal FLAG tag

   Depositor

   Shaun Coughlin

   Article

   Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

   Insert

   Par1 (F2R Human)
   

   Tags

   FLAG and bovine prolactin signal sequence (MDSKGS…

   Expression

   Mammalian

   Mutation

   Deletion of aa1-39 (native signal peptide)

   Promoter

   SV40

   Available Since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEFL-EGFP-TEADiv2

    Plasmid

    #228872

    Purpose

    Expression of optimized TEAD inhibitor (TEADiv2): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    TEADiv2
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3543 pMIG Bcl-XL mut8

    Plasmid

    #8792

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G138A, R139L, I140N

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

12. IDG_TMEM63B_OE_1

    Plasmid

    #161682

    Purpose

    Overexpresses TMEM63B

    Depositor

    Mike McManus

    Insert

    TMEM63B (TMEM63B Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKH3-human RSK1

    Plasmid

    #13841

    Depositor

    John Blenis

    Article

    Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

    Insert

    RSK1 (RPS6KA1 Human)
    

    Tags

    3x HA

    Expression

    Mammalian

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. H2B-GFP-Aurora B WT

    Plasmid

    #184042

    Purpose

    Expresses H2B-GFP fused to Aurora B WT

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    AURKB (AURKB Human)
    

    Tags

    EGFP and H2B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-3-VEGFR3

    Plasmid

    #119230

    Purpose

    Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.

    Depositor

    Kari Alitalo

    Insert

    VEGFR3 (FLT4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-Ezrin-C-14

    Plasmid

    #55042

    Purpose

    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    NM_003379.4 (EZR Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCINEO-3xFlag-hRIPK3

    Plasmid

    #112495

    Purpose

    Mammalian expression of 3xFlag-hRIPK3

    Depositor

    Junying Yuan

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Spike-VLP

    Plasmid

    #173922

    Purpose

    Expresses virus-like particles based upon the original SARS-Cov-2

    Depositor

    Andreas Stuermer

    Inserts

    SARS-2 Spike Protein (S SARS-COV-2, Human, Synthetic)
    SARS-Cov-2 E protein (E SARS-COV-2, Human, Synthetic)
    SARS-Cov-2 M Protein (M SARS-COV-2, Human, Synthetic)
    

    Tags

    no tags, native protein

    Expression

    Bacterial and Mammalian

    Promoter

    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

    Available Since

    April 11, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. AAV SYN PSAM4 GlyR IRES EGFP

    Plasmid

    #119742

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti GFP-AMOT p130

    Plasmid

    #32828

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

    Insert

    AMOT p130 (AMOT Human)
    

    Use

    Lentiviral

    Tags

    GFP hAMOT

    Expression

    Mammalian

    Available Since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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