We narrowed to 11,749 results for: ARIA;

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pETDUET-GST AO7REwt

Plasmid

#213935

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

AO7 cloned into the first multiple cloning site o…

Promoter

T7 promoter-1

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pQE80L-SH3

Plasmid

#160331

Purpose

This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.

Depositor

Sang Taek Jung

Article

Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

Insert

SH3

Tags

6xHis tag

Expression

Bacterial

Promoter

T5

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-R203K

Plasmid

#177951

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203K) (N )

Expression

Mammalian

Mutation

R203K

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV iGluu

Plasmid

#105832

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR S72T variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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AM77_pU6.Sa-gRNA.CLYBL

Plasmid

#199237

Purpose

Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

S. aureus gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-KKHSaCas9-BlastR

Plasmid

#107189

Purpose

Confers constitutive expression of KKH SaCas9.

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Insert

KKH SaCas9 (NEWENTRY )

Expression

Bacterial

Mutation

E782K/N968K/R1015H

Promoter

Chicken ß-Actin

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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VEGF binding scFv G6

Plasmid

#111716

Purpose

Yeast Surface Display of G6 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6

Tags

cmyc tag

Expression

Yeast

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pOP/SSAT

Plasmid

#140291

Purpose

Expresses human SSAT in bacterial cells

Depositor

Saverio Bettuzzi, Gaetano Marverti

Article

Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pII pm

Plasmid

#218099

Purpose

Phagemid that expresses LacI, and pII under the Lac promoter

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Insert

pII

Expression

Bacterial

Promoter

Lac promoter

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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CABE-T2.19

Plasmid

#193265

Purpose

Expression of CABE-T2.19 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T2.19

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CoV2-N-G204R

Plasmid

#177953

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (G204R) (N )

Expression

Mammalian

Mutation

G204R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR1 I280A

Plasmid

#84495

Purpose

overexpression of CASTOR1 I280A mutant

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 I280A (GATSL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

I280A

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
DEL-pHG165a

Plasmid

#90064

Purpose

LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

DEL

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean-LaminB1-10

Plasmid

#55380

Purpose

Localization: Nuclear Envelope, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

LaminB1 (LMNB1 Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
ATG9A-2STrep

Plasmid

#210359

Purpose

Expresses ATG9A fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

ATG9A (ATG9A Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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p3XFlag-Mettl4

Plasmid

#86665

Purpose

Expresses murine Mettl4 containing N-terminal 3XFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Mettl4 (Mettl4 Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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LacI-11/109-pHG165c

Plasmid

#90059

Purpose

Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI-11/109

Expression

Bacterial

Mutation

109

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS-CoV-2 Nsp3TM

Plasmid

#204206

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx1

Expression

Mammalian

Mutation

none, from obtained clone

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RVQ

Plasmid

#200187

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RVQ

Use

CRISPR; Gateway compatible lbcas12a-rv entry clone

Tags

NLS

Expression

Plant

Mutation

G146R, R182V, E795Q

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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