We narrowed to 11,247 results for: SHA

1. pfast bac-flag Fbxl3

   Plasmid

   #51894

   Purpose

   Backbone plasmid is pfastBacHTa from invitrogen

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   Fbxl3 (Fbxl21 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pGEMHE-Trim21-OLLAS

   Plasmid

   #237438

   Purpose

   Expresses mouse Trim21 ubiquitin ligase tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

   Insert

   Trim21 (Trim21 Mouse)
   

   Use

   Tags

   OLLAS

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLuc-N-HiBiT

   Plasmid

   #207117

   Purpose

   Bacterially expressed HiBiT-FLuc for Ni-NTA purification

   Depositor

   James Inglese

   Article

   Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

   Insert

   luciferin 4-monooxygenase
   

   Use

   Tags

   HiBiT Tag and His-tag with thrombin site

   Expression

   Bacterial

   Mutation

   Codon optmization for expression in BL21DE3

   Promoter

   T7 Promotor

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLuc-C-HiBiT

   Plasmid

   #207118

   Purpose

   Bacterially expressed FLuc-HiBiT for Ni-NTA purification

   Depositor

   James Inglese

   Article

   Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

   Insert

   luciferin 4-monooxygenase
   

   Use

   Tags

   HiBiT Tag and His-tag with thrombin site

   Expression

   Bacterial

   Mutation

   Codon optmization for expression in BL21DE3

   Promoter

   T7 Promotor

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLuc-C-NLuc

   Plasmid

   #234566

   Purpose

   Bacterially expressed FLuc-C-NLuc for Ni-NTA purification

   Depositor

   James Inglese

   Article

   Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

   Insert

   Firefly luciferase
   

   Use

   Tags

   NanoLuciferase-thrombin site-10xHis and T7 gene 1…

   Expression

   Bacterial

   Mutation

   Codon optmization for expression in BL21DE3

   Promoter

   T7

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH47822_Myc-Sr62TK

   Plasmid

   #233536

   Purpose

   Expresses Myc-Sr62TK in planta

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   Sr62TK
   

   Use

   Tags

   Myc tag

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

   Plasmid

   #234883

   Purpose

   non-targeting CRISPRi control

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   L1HS gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

   Plasmid

   #234882

   Purpose

   L1HS-silencing plasmid (CRISPRi gRNA1)

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   L1HS-silencing plasmid (CRISPRi gRNA1)
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6-CFAPoptim-KAN

   Plasmid

   #221128

   Purpose

   Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS415-TEF1pr-SEC63-FAPoptim

    Plasmid

    #221116

    Purpose

    FAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    FAPoptim-SEC63
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRDA_052_HygroR

    Plasmid

    #228937

    Purpose

    Plasmid for cloning of custom gRNA using EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS415-TEF1pr-SEC61-FAPoptim

    Plasmid

    #221115

    Purpose

    FAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC61-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS413-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221095

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-ImpBeta1-mEosEM

    Plasmid

    #218933

    Purpose

    Express ImpBeta1 (human) with mEosEM fused at the C-terminus.

    Depositor

    Siegfried Musser

    Article

    Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

    Insert

    Importin Beta (KPNB1 Human)
    

    Use

    Tags

    6xHis and mEos4b (EosEM)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Start Stuffer TurboID V5 T2A BFP

    Plasmid

    #233257

    Purpose

    Expression of BFP-tagged vector for Turbo ID experiments

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    TurboID V5 T2A BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS413-TEF1pr-ANP1-FAPoptim

    Plasmid

    #221120

    Purpose

    FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    ANP1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS413-TEF1pr-ERG6-FAPoptim

    Plasmid

    #221121

    Purpose

    FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    ERG6-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS413-TEF1pr-PMA1-FAPoptim

    Plasmid

    #221122

    Purpose

    FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    PMA1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS413-TEF1pr-SEC7-FAPoptim

    Plasmid

    #221124

    Purpose

    FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC7-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS413-TEF1pr-VPH1-FAPoptim

    Plasmid

    #221125

    Purpose

    FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    VPH1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge