We narrowed to 5,906 results for: pcDNA 3.1

1. pcDNA3.1-GGamma10-GFP2

   Plasmid

   #166779

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma10-GFP2 (GNG10 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-SARS-Spike

   Plasmid

   #145031

   Purpose

   Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   SARS-CoV Spike
   

   Use

   Tags

   C9 and CD5 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1-Zeo-mEGFP

   Plasmid

   #214145

   Purpose

   Expresses the monomeric EGFP in eukaryotic cells

   Depositor

   Bertrand Collet

   Article

   Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A207K L221K F223R

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-GGamma8-GFP2

   Plasmid

   #140990

   Purpose

   Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma8-GFP2 (GNG8 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-HDAC2-H141A

   Plasmid

   #115346

   Purpose

   Expresses mouse HDAC2 cDNA with H141A point mutation

   Depositor

   Javier Gonzalez-Maeso

   Article

   Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

   Insert

   Histone deacetylase 2 (Hdac2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H141A

   Promoter

   CMV

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-GGamma13-GFP2

   Plasmid

   #140992

   Purpose

   Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma13-GFP2 (GNG13 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-2xFLAG-SREBP-2

   Plasmid

   #26807

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   L7V, G76S, K395R and I482L

   Promoter

   CMV

   Available since

   July 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-KIT-V5/HIS

   Plasmid

   #201986

   Purpose

   expression of human KIT receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   KIT receptor tyrosine kinase (KIT Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CHRFAM7A-YFP

   Plasmid

   #62634

   Purpose

   mammalian expression of human duplicated Alpha7 (CHRFAM7A) with YFP fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRFAM7A (CHRFAM7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   YFP inserted in M3-M4 loop

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-SC35-cMyc

    Plasmid

    #44721

    Depositor

    Kathleen Scotto

    Article

    Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

    Insert

    SC35 (SRSF2 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-Gβγ-BERKY3

    Plasmid

    #158219

    Purpose

    BRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-TRKB-V5/HIS

    Plasmid

    #205614

    Purpose

    Expression of human TRKB receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TRKB receptor tyrosine kinase (NTRK2 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 myc-RNF114

    Plasmid

    #58295

    Purpose

    mammalian expression of myc tagged RNF114

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-MS2-HA-HshnRNPC1_Z

    Plasmid

    #148114

    Purpose

    Mammalian Expression of HshnRNPC1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPC1 (HNRNPC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FLAG-OTUB1 WT-pcDNA3.1

    Plasmid

    #118209

    Purpose

    Expresses FLAG-OTUB1 WT in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

    Plasmid

    #114595

    Purpose

    Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

    Depositor

    Brian Chow

    Article

    Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

    Insert

    BcLOV4
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1_iSH2-pMag(3x)-iRFP

    Plasmid

    #67304

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    iSH2-pMag(3x)-iRFP (PIK3R1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-FGFR4-V5/HIS

    Plasmid

    #201109

    Purpose

    expression of human FGFR4 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-Hyg-RFPKDEL

    Plasmid

    #138660

    Purpose

    Reporter plasmid expressing the ER-located RFP

    Depositor

    Bertrand Collet

    Article

    Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

    Insert

    RFP-KDEL
    

    Use

    Tags

    KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1_iSH2-pMagFast2(3x)-iRFP

    Plasmid

    #67298

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    iSH2-pMagFast2(3x)-iRFP (PIK3R1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge