We narrowed to 1,958 results for: cp

1. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

   Plasmid

   #91818

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1355 and lysine 1435 to alanines; …

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMY-CD90.2-P2A-BlastDYK

   Plasmid

   #163349

   Purpose

   Retroviral vector to express the surface marker CD90.2 and a DYK-tagged blasticidin resistance marker

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD90.2 (Thy1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMX-mPGK-CD3-P2A-CD90.2

   Plasmid

   #163337

   Purpose

   Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the mPGK promoter

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD3
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   mPGK

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMX-hFTH1-CD3-P2A-CD90.2

   Plasmid

   #163335

   Purpose

   Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the hFTH1 promoter

   Depositor

   Teunis Geijtenbeek, Louis Ates

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD3
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   hFTH1

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CBSH3+ shRNA-2

   Plasmid

   #160068

   Purpose

   Knock Down SH3+ Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CBSH3+ shRNA-1

   Plasmid

   #160066

   Purpose

   Knock Down SH3+ Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CBSH3- shRNA2

   Plasmid

   #160210

   Purpose

   Knock Down MTLSH3- Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Human, Rat)
   

   Use

   RNAi

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CBSH3- shRNA3

   Plasmid

   #160212

   Purpose

   Knock Down MYDSH3- Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Human, Rat)
   

   Use

   RNAi

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CBSH3- shRNA1

   Plasmid

   #160208

   Purpose

   Knock Down MQWSH3- Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Human, Rat)
   

   Use

   RNAi

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

    Plasmid

    #123354

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    cp-moxCerulean3-moxCerulean3-FLARE-D1ER
    

    Tags

    C-terminal ER retention signal (KDEL), cp-moxCeru…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. U6-FnCas12a-TLR-MCV1-gRNA

    Plasmid

    #117412

    Purpose

    FnCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    FnCas12a gRNA targeting TLR 2.0
    

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pet151D-TOPO-BROX 1-411; K141E

    Plasmid

    #89865

    Purpose

    Expresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    mutated lysine 141 to glutamic acid

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pet151D-TOPO-BROX 1-411; L212D

    Plasmid

    #89864

    Purpose

    Expresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    mutated leucine 212 to aspartic acid

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-CHMP4A (205-222) L217A (pGEX2T(TEV))

    Plasmid

    #80638

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Leucine 217 to Alanine

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-CHMP4A (205-222) W220A (pGEX2T(TEV))

    Plasmid

    #80637

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Trp 220 to Ala

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-CHMP4A (205-222) L214A (pGEX2T(TEV))

    Plasmid

    #80639

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Leucine 214 to Alanine

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)

    Plasmid

    #61935

    Purpose

    Expression of hRPN13 (285-407) (Q337A/Q338A) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hRPN13 (285-407) (ADRM1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Construct starts at residue 285, residues Q337 an…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS

    Plasmid

    #61940

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156) GSGS (NFRKB Human)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156, residues 97-100 (N…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

    Plasmid

    #61941

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-117) (NFRKB Human)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 117, stop codon introdu…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pMXs-IP-mCherry-TOSI

    Plasmid

    #172496

    Purpose

    mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    PDCD4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    Mcherry

    Expression

    Mammalian

    Promoter

    MLV LTR

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. PEmaxNG-_2star_IVT

    Plasmid

    #227678

    Purpose

    plasmid for SpCas9-NG-PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    NG-PEmax_2_star
    

    Use

    CRISPR

    Tags

    no

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. sFlt1-i13 V5

    Plasmid

    #86044

    Purpose

    human Soluble Flt1 isoform i13 (terminates in intron 13)

    Depositor

    Christie Thomas

    Article

    Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

    Insert

    human soluble Flt1 isoform i13 (FLT1 Human)
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    isoform i13 (terminates in intron 13)

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. sFlt1-e15a V5

    Plasmid

    #86045

    Purpose

    human Soluble Flt1 isoform e15a (terminates in exon 15a)

    Depositor

    Christie Thomas

    Article

    Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

    Insert

    human soluble Flt1 isoform e15a (FLT1 Human)
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    isoform e15a (terminates in exon 15a)

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. M23: pHR-SFFVp-FUSN::mCherry::SspB

    Plasmid

    #122148

    Purpose

    N terminal domain of FUS (1-214) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FUS (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-214

    Promoter

    SFFV

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

    Plasmid

    #122147

    Purpose

    Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Lentiviral

    Tags

    NLS-iLID-GFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXs-IP-mVenus-TOSI

    Plasmid

    #172491

    Purpose

    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Pdcd4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    MLV LTR

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. M43: pHR-SFFVp-iLID::EGFP::FTH1

    Plasmid

    #122149

    Purpose

    Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Lentiviral

    Tags

    NLS-iLID-GFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

    Plasmid

    #122668

    Purpose

    C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    HNRNPA1 (HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-SspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 186-230

    Promoter

    SFFV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTMB352_ZFcharm_Kv1_split-inteins

    Plasmid

    #220848

    Purpose

    Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv2
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTMB301_ZFcharm_Kv1_Prnp_SPM

    Plasmid

    #220840

    Purpose

    AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv1
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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