Addgene

We narrowed to 2,289 results for: puc19

Showing: 961 - 990 of 2289 results

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pHelper_AcCAST

Plasmid

#127923

Purpose

Expresses AcCAST and a crRNA targeting PSP1

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

AcTnsB, AcTnsC, AcTniQ, AcCas12k

Use

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPRainbow-DONOR1

Plasmid

#75398

Purpose

CRISPRainbow-DONOR1

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Cm(R)-CcdB

Use

CRISPR

Tags

no

Expression

Mutation

Promoter

Bacteria

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_D17

Plasmid

#84611

Purpose

Introduces DSB at D17 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLacZa-GFP

Plasmid

#86440

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

LacZalpha-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

LacZa-sfGFP

Expression

Mutation

Promoter

Plac

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pT1UciMP

Plasmid

#62215

Purpose

UAS transgenesis vector

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

UAS-E1b

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_A08

Plasmid

#84610

Purpose

Introduces DSB at A08 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGGB003

Plasmid

#48821

Purpose

Provides a default dummy as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

B-Dummy

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pT2/SVNeo

Plasmid

#26553

Depositor

Perry Hackett

Article

Cui et al J Mol Biol. 2002 May 17. 318(5):1221-35.

Insert

pT2/SVNeo

Use

Tags

Expression

Bacterial

Mutation

added the Rmut(80, 87, d101)

Promoter

Available since

March 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-miRFP670-pA_U6-BbsI (pE059)

Plasmid

#149353

Purpose

Mammalian expression vector for miRFP670

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

miRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_XPR2

Plasmid

#84609

Purpose

Introduces DSB at XPR2 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_AXP

Plasmid

#84608

Purpose

Introduces DSB at AXP locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGGF005

Plasmid

#48846

Purpose

Provides a hygromycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pUBQ10::HygromycinR::tOCS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMH220

Plasmid

#122836

Purpose

dCas9 with GreenGate B-C flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Contains D10A and H840A mutations

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGGF007

Plasmid

#48847

Purpose

Provides a kanamycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::KanamycinR::tNOS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGD002

Plasmid

#48834

Purpose

Provides a default dummy as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

D-Dummy

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGA006

Plasmid

#48816

Purpose

Provides the UBQ10 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 promoter

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCsy4

Plasmid

#44252

Depositor

Stanley Qi, Adam Arkin

Article

Qi et al Nat Biotechnol. 2012 Oct;30(10):1002-6.

Insert

csy4 gene

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLtetO-1

Available since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGGF003

Plasmid

#48844

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::D-AlanineR::tMAS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE001

Plasmid

#48839

Purpose

Provides the rbcS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

rbcS terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-UnaG-pA (pE091)

Plasmid

#149354

Purpose

Mammalian Expression vector for UnaG

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCJ155

Plasmid

#175054

Purpose

psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Tags

Expression

Mutation

Promoter

rbcLXS

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGGA004

Plasmid

#48815

Purpose

Provides the 35S promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

35S promoter

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

internal BsaI recognition site removed by substit…

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pH1v1

Plasmid

#60244

Purpose

gRNA expression by the H1 promoter

Depositor

Donald Zack

Article

Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE009

Plasmid

#48841

Purpose

Provides the UBQ10 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGG001

Plasmid

#48850

Purpose

Provides an F-H adapter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

F-H adapter - short

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCfB8742

Plasmid

#126908

Purpose

Template for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolytica

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

pGPD1 and pFBA1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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ATM_N_FS_v2_Donor

Plasmid

#214815

Purpose

N-terminal tagging of human ATM with 3xFLAG_Twin_Strep

Depositor

Yannick Doyon

Insert

3xFLAG_2xStrep for tagging ATM at the N-terminus (ATM Human, Synthetic)

Use

CRISPR

Tags

3xFLAG_2xStrep

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> :dTomato:IRES:Puro

Plasmid

#110832

Purpose

Expresses dTomato (dT) red fluorescent reporter.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

dTomato Tag

Use

Non-viral gene expression vector

Tags

IRES

Expression

Mutation

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMAZ-donor

Plasmid

#112359

Purpose

CRISPR donor plasmid to tag human transcription factor MAZ with GFP

Depositor

Kevin White

Insert

MAZ homology arms flanking EGFP-IRES-Neo cassette (MAZ Human)

Use

CRISPR

Tags

Expression

Mutation

hg38:16:29809765:G>A

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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AP575-1

Plasmid

#71277

Purpose

Expresses 3Xflag::tagRFP::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagRFP

Use

Tags

3xFlag and myc

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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