We narrowed to 1,429 results for: tk

1. pFusX1-16

   Plasmid

   #63234

   Purpose

   Encodes RVD positions 1-3 in TALEN assembly

   Depositor

   Stephen Ekker

   Article

   Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

   Insert

   NI NG NG
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   none

   Promoter

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pLN428 (FuGW-G5p-mKate2-1Pe )

   Plasmid

   #105184

   Purpose

   Module 3 - GAL4 synthetic promoter (with five binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G5p-mKate2-1Pe
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLN434 (FuGW-G14p-mKate2-1Pe )

   Plasmid

   #105188

   Purpose

   Module 3 - GAL4 synthetic promoter (with fourteen binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G14p-mKate2-1Pe
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAMHDAdGT8-4

   Plasmid

   #26421

   Depositor

   Kohnosuke Mitani

   Insert

   HSV-TK, mCMV-b-gal
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCIHDAdGT8-3

   Plasmid

   #26525

   Depositor

   Kohnosuke Mitani

   Insert

   HSV-TK, mCMV-b-gal
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET3a-hUBA1

   Plasmid

   #63571

   Purpose

   Expression of human UBA1 tagged with His

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBA1 (UBA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Isoform 2 (1018 amino acids)

   Promoter

   T7

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPolI_SARS-CoV-2_NLuc

   Plasmid

   #183953

   Purpose

   Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

   Depositor

   AJ te Velthuis

   Article

   Vial et al bioRxiv 2021.06.28.450211

   Insert

   SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mammalian Pol I

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PGK1p-Csy4-pA (Construct 2)

   Plasmid

   #55196

   Purpose

   Expresses Csy4 in Mammalian cells for site-specific RNA processing

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   Csy4/Cas6
   

   Use

   Synthetic Biology

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG496

   Plasmid

   #226750

   Purpose

   Expresses exogenous MTCH2

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MTCH2 (MTCH2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-CAG-FLEX-BFP2-(mCherry)’

    Plasmid

    #115234

    Purpose

    Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    mTagBFP2 and mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG397

    Plasmid

    #226733

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    OMP25 (SYNJ2BP Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMM534

    Plasmid

    #112530

    Purpose

    pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Insert

    luxCDABE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PL(HrtO)

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGPA1

    Plasmid

    #115476

    Purpose

    Generation of mariner-based transposon mutant libraries in Enterococcus faecium

    Depositor

    Willem van Schaik

    Article

    Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

    Plasmid

    #216533

    Purpose

    An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

    Depositor

    Yuki Kambe

    Article

    Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

    Insert

    axon-jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    GAP43

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hCEP120-EGFP

    Plasmid

    #50382

    Purpose

    Over-expression of EGFP-tagged hCEP120

    Depositor

    Tang Tang

    Article

    Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

    Insert

    Human CEP120 (CEP120 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NDV-HN-SpyTag

    Plasmid

    #160000

    Purpose

    Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    NDV-HN-SpyTag
    

    Use

    Tags

    His6-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta-actin promoter

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMS_366

    Plasmid

    #182131

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Use

    Tags

    Expression

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Promoter

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGM8

    Plasmid

    #220166

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    TSPO (TSPO Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac1-Ls-AChBP

    Plasmid

    #63126

    Purpose

    Expression of Lymnaea stagnalis AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpyTag003-OspC

    Plasmid

    #159998

    Purpose

    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag003-OspC (20-209) C129S
    

    Use

    Tags

    His6-tag and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    C129S mutation improves expression level.

    Promoter

    T7

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFastBac1-Ac-AChBP

    Plasmid

    #63128

    Purpose

    Expression of Aplysia californica AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pETNKI-his-SUMO-RNF168ring (1-189)

    Plasmid

    #63570

    Purpose

    Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    6xHis and SUMO3

    Expression

    Bacterial

    Mutation

    Ring domain only (residues 1-189)

    Promoter

    T7

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)

    Plasmid

    #105201

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-IL12-P2A-CCL21-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3.1-Flag-H2A K13R

    Plasmid

    #63566

    Purpose

    Expression of human H2A with K13R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine at 13 is now Arginine

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pCDNA3.1-Flag-H2A K15R

    Plasmid

    #63567

    Purpose

    Expression of human H2A with K15R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine at 15 is now Arginine

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

26. EGFP in pDBEcR-pIND(SP1)-DD/Bla

    Plasmid

    #201559

    Purpose

    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.

    Depositor

    Randy Poon

    Article

    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

    Insert

    DBEcR and EGFP
    

    Use

    Unspecified; Sleeping beauty transposon

    Tags

    DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and pIND(SP1)

    Available Since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFP in pDBEcR-pIND(SP1)-W/Bla

    Plasmid

    #201558

    Purpose

    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

    Depositor

    Randy Poon

    Article

    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

    Insert

    DBEcR and EGFP
    

    Use

    Unspecified; Sleeping beauty transposon

    Tags

    DBEcR-myc and FLAG-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and pIND(SP1)

    Available Since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

    Plasmid

    #55199

    Purpose

    CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    ECFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOP459

    Plasmid

    #85933

    Purpose

    Encodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 2G4 monoclonal antibody
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOP462

    Plasmid

    #85935

    Purpose

    Encodes the Anti-Ebola 13C6 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 13C6 monoclonal antibody
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge