We narrowed to 1,409 results for: 4-Oct

1. pET51b(+)_MBP-nLucCP-67/68

   Plasmid

   #113922

   Purpose

   Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   MBP-NanoLucCP-67/68
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLC-255

   Plasmid

   #124292

   Purpose

   Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

   Depositor

   Swaine Chen

   Article

   Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

   Insert

   vsfGFP-9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pINTO-N-SA

   Plasmid

   #61723

   Purpose

   Inducible expression of proteins fused to an N-terminal monomeric streptavidin tag (mSA)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Type

   Empty backbone

   Use

   Tags

   mSA

   Expression

   Mammalian

   Mutation

   Promoter

   T-REx (CMV + 2xTetO2)

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRRL-5xTetO-pEF-H2B-Citrine-SV40

   Plasmid

   #186966

   Purpose

   Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   5xTetO-pEF-H2B-Citrine-SV40
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   5xTetO-pEF-1alpha

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3 MDM2 1-400

   Plasmid

   #45754

   Depositor

   Robert Lefkowitz

   Article

   Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

   Insert

   MDM2 1-400 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 1-400, lacks catalytic RING domain

   Promoter

   CMV

   Available since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pRK5-mFzd4-1D4

   Plasmid

   #42266

   Depositor

   Jeremy Nathans, Chris Garcia

   Article

   Yu et al Development. 2012 Dec 1;139(23):4383-94.

   Insert

   Frizzled 4 (Fzd4 Mouse)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

7. 1000.pCCLsin.cPPT.hPGK.Brn2.MIRT124_x4 Xma_lost.WPRE

   Plasmid

   #67295

   Purpose

   Induce human iN conversion from fibroblast

   Depositor

   Malin Parmar

   Article

   Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

   Insert

   Brn2 (Pou3f2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSCVneo-HOXB3-FLAG

   Plasmid

   #74508

   Purpose

   Retroviral expression of HOXB3

   Depositor

   Lars Rönnstrand

   Article

   Lindblad et al Biochem Biophys Res Commun. 2015 Nov 27;

   Insert

   HOXB3 (HOXB3 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   unknown

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. LDB204

   Plasmid

   #185426

   Purpose

   Bacterial expression of FG-repeat domain of NUP1 nucleoporin as GST-Nup1-Rep fusion

   Depositor

   Laura Davis, Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 FXFG-repeats domain expressed

   Promoter

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET51b(+)_S-Luc_CLIP

    Plasmid

    #113923

    Purpose

    Plasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc_CLIP
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDEST15-Nedd4.2F823A

    Plasmid

    #165104

    Purpose

    bacterial expression of a GST fusion of Human Nedd4.2d F823A

    Depositor

    Arthur Haas

    Article

    Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

    Insert

    Nedd4.2d F823A (NEDD4L Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    F823A; mutation for destabilization of trimer fo…

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD

    Plasmid

    #73306

    Purpose

    Expresses mutant human PUM1-HD

    Depositor

    Traci Hall

    Article

    Wang et al Nat Methods. 2009 Nov;6(11):825-30. doi:

    Insert

    human PUM1 Pumilio homology domain (PUM1 Human)
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    Q867E-Q939E-C935S-Q1011E-C1007S

    Promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

13. RcSxph

    Plasmid

    #194018

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. E42_control_NGFR

    Plasmid

    #189800

    Purpose

    Retroviral delivery of control guide RNA

    Depositor

    Ellen Rothenberg

    Article

    Hosokawa et al Nat Immunol. 2018 Dec;19(12):1427-1440.

    Insert

    Luciferase gRNA
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KBB394

    Plasmid

    #185086

    Purpose

    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Nma111 KKR 9-11 AAA; KRK 28-30 AAA

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX6P1-optimizedNedd4.2F823D

    Plasmid

    #165105

    Purpose

    bacterial expression of a GST fusion of Human Nedd4.2d F823D

    Depositor

    Arthur Haas

    Article

    Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

    Insert

    Nedd4.2d F823D (NEDD4L Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression; F823D; …

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET51b(+)_MBP-nLucCP-65/68

    Plasmid

    #113921

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-65/68
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET51b(+)_MBP-nLucCP-65/66

    Plasmid

    #113920

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-65/66
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MIP 247 CoMiP 4in1 with shRNA p53

    Plasmid

    #63726

    Purpose

    To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

    Depositor

    Mark Kay, Joseph Wu

    Article

    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

    Insert

    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
    

    Use

    Tags

    IRES-dtomato

    Expression

    Mammalian

    Mutation

    codon-optimised

    Promoter

    SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pW17

    Plasmid

    #158207

    Purpose

    2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSLQ10704

    Plasmid

    #183957

    Purpose

    U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    SKSKSO array
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSLQ10871

    Plasmid

    #183962

    Purpose

    U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    dCas12a(wildtype)
    poly-crRNA array targeting Sox2-Klf4-Oct4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG and U6

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215327

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    MUC5AC Promoter
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MUC5AC

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAM4787

    Plasmid

    #120088

    Purpose

    Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lamp1-mCherry-RUSH

    Plasmid

    #202799

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    Lamp1 (Lamp1 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215328

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    FOXJ1 Promoter
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FOXJ1

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJRoC30_Th3.14

    Plasmid

    #188068

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 4 FuncLib mutations, described in publ…

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSEMS-SDHB-Halo7Tag

    Plasmid

    #108973

    Purpose

    Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

    Depositor

    Karin Busch

    Article

    Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

    Insert

    succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
    

    Use

    Tags

    Halo7-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFRT/FLAG/HA-DEST EIF2C4

    Plasmid

    #19890

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Eukaryotic translation initiation factor 2C, 4 (AGO4 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMKO.1-shMki67

    Plasmid

    #160958

    Purpose

    Mki67 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Mki67 (Mki67 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMKO.1-shPrc1

    Plasmid

    #160959

    Purpose

    Prc1 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Prc1 shRNA (Prc1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

    Plasmid

    #160635

    Purpose

    TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJRoC30_Th3.1

    Plasmid

    #188065

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 4 FuncLib mutations, described in publ…

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFastBac1-NpSxph

    Plasmid

    #193097

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Nanorana parkeri saxiphilin
    

    Use

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFastBac1-NpSxphI559Y

    Plasmid

    #193099

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Nanorana parkeri saxiphilin I559Y
    

    Use

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    I559Y

    Promoter

    polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFastBac1-OsSxph

    Plasmid

    #193100

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Oophaga sylvatica saxiphilin
    

    Use

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. RcSxph E540A

    Plasmid

    #194019

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. RcSxph E540D

    Plasmid

    #194020

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540D

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RcSxph E540Q

    Plasmid

    #194021

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540Q

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. RcSxph Y558A

    Plasmid

    #194022

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge