We narrowed to 2,905 results for: BFP

1. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

   Plasmid

   #128762

   Purpose

   Expresses DIS3L2 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   DIS3L2 sgRNA #1 (DIS3L2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

   Plasmid

   #128760

   Purpose

   Expresses PARN gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PARN sgRNA #2 (PARN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

   Plasmid

   #128758

   Purpose

   Expresses PAPD4 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PAPD4 sgRNA #3 (TENT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

   Plasmid

   #128756

   Purpose

   Expresses PAPD4 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PAPD4 sgRNA #1 (TENT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

   Plasmid

   #128757

   Purpose

   Expresses PAPD4 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PAPD4 sgRNA #1 (TENT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

   Plasmid

   #117137

   Purpose

   Expression vector encoding BFP-BSD-Nkx2-5

   Depositor

   Kosuke Yusa

   Article

   Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

   Insert

   Nkx2-5 CDS (Nkx2-5 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   modified BFP

   Promoter

   human U6 promoter, human EF1a promoter

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBS1502 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaCTD]

   Plasmid

   #133326

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of CTD in full-length TDP43

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

   Plasmid

   #122151

   Purpose

   Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   dCas9 fused to SunTag(24x)
   

   Use

   Lentiviral

   Tags

   2xNLS-P2A-BFP and NLS

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

   Plasmid

   #188777

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   EF1a

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

    Plasmid

    #188767

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)

    Plasmid

    #180587

    Purpose

    FdCd

    Depositor

    Won Do Heo

    Article

    Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

    Insert

    Cre
    

    Use

    AAV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

    Plasmid

    #219548

    Purpose

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
    

    Expression

    Mammalian

    Mutation

    The (SAGG)x4 linker between GAL4(1-65) and the PC…

    Promoter

    CMVd1

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

    Plasmid

    #170273

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
    

    Expression

    Mammalian

    Mutation

    See Comments

    Promoter

    CMVd1

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

    Plasmid

    #176027

    Purpose

    construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    rtTA3
    Luc2
    mtagBFP
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158653

    Purpose

    making transgenics expressing a UAS driven mCherry and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158652

    Purpose

    making transgenics expressing a UAS driven eGFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-GAL4-IL10-IRES-mCherry-PGK-BFP

    Plasmid

    #223209

    Purpose

    Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL10 (IL10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200402

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM]

    Plasmid

    #133325

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of RRM in full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221075

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221076

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221077

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221079

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD]

    Plasmid

    #133324

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of NTD in full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

    Plasmid

    #159798

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

    Plasmid

    #216478

    Purpose

    lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    YAP5SA
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Mutation

    S61A, S109A, S127A, S164A, S381A

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

    Plasmid

    #216479

    Purpose

    lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    wtYAP (YAP1) (YAP1 Human)
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

    Plasmid

    #233445

    Purpose

    PiggyBac donor plasmid to overexpress a full length ADAR2

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    ADAR2-T2A-PuroR-P2A-BFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1a

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid

    #187956

    Purpose

    FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-hHDAC4-SpdCas9-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

    Plasmid

    #216477

    Purpose

    lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158651

    Purpose

    making transgenics expressing a UAS driven YFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid

    #187957

    Purpose

    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid

    #187953

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

    Plasmid

    #228252

    Purpose

    pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    SpCas9 P23H sgRNA, mTagBFP2
    

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200403

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

    Plasmid

    #164038

    Purpose

    Expression of dCas9-14xGFP11 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    dCas9-14xGFP11
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    dSV40

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP

    Plasmid

    #216480

    Purpose

    lentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    TAZ4SA (WWTR1 Human)
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Mutation

    S66A, S89A, S117A, S311A

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

    Plasmid

    #118594

    Purpose

    Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    sgRNA/Puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204722

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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40. pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

    Plasmid

    #137994

    Purpose

    Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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