We narrowed to 2,097 results for: Pam

1. PL452-FGF8-LA-p2a-tdTomato-hGHpA-PGK-neoR-FGF8-RA

   Plasmid

   #191687

   Purpose

   For knocking p2a-tdTomato into FGF8 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   FGF8 homology arms and p2a-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PL452-PTPRO-LA-p2a-EGFP-hGHpA-PGK-neoR-PTPRO-RA

   Plasmid

   #191689

   Purpose

   For knocking p2a-EGFP into PTPRO locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   PTPRO homology arms and p2a-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKM5

   Plasmid

   #154166

   Purpose

   Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel2 (long isoform)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKM28

   Plasmid

   #154165

   Purpose

   Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel1A
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAA2

   Plasmid

   #154162

   Purpose

   Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   16 wt NF-κB sites were replaced with Rel1A-specif…

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAA1

   Plasmid

   #154161

   Purpose

   Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   12 wt NF-κB sites were replaced with Rel1A-specif…

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKM62

   Plasmid

   #154160

   Purpose

   Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   4 wt NF-κB sites were replaced with Rel2-specific…

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKM59

   Plasmid

   #154158

   Purpose

   Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Enhancer for the Drosophila melanogaster Metchnik…

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKM52

   Plasmid

   #154155

   Purpose

   Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB site

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Drosophila melanogaster minimal Hsp70 promoter wi…

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKM51

    Plasmid

    #154154

    Purpose

    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB site

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Drosophila melanogaster minimal Hsp70 promoter wi…

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmCherry-Synaptojanin-1-145

    Plasmid

    #223563

    Purpose

    Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

    Depositor

    Pietro De Camilli

    Article

    Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Use

    Tags

    His and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PiggyBac-TetO-Ascl1-neomycin

    Plasmid

    #176482

    Purpose

    TetO-Ascl1 in a PiggyBac vector with neomycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

    Plasmid

    #184635

    Purpose

    Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCWori cytochrome P450-BM3h 9D7

    Plasmid

    #61308

    Purpose

    Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

    Depositor

    Alan Jasanoff

    Article

    Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

    Insert

    Cytochrome P450-BM3 heme domain 9D7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T268A, I263A, T438V, A328G

    Promoter

    Tac

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV.PGK.mFoxa2

    Plasmid

    #33014

    Depositor

    Malin Parmar

    Article

    Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

    Insert

    Forkhead box A2 (Foxa2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H10L

    Promoter

    Available since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-CAG-SPOTlight-U2GCR

    Plasmid

    #164819

    Purpose

    Reporter to measure ISR state-dependent protein synthesis

    Depositor

    Nicole Calakos

    Article

    Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

    Insert

    SPOTlight-U2GCR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-DIO-EF1α-mKate2-biPAC

    Plasmid

    #169127

    Purpose

    AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Insert

    mKate2-biPAC
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    codon optimized to mouse

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-pTH-iCre:EGFP-WPREpA

    Plasmid

    #213142

    Purpose

    Truncated rat TH promoter expressing iCre fused to EGFP

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA

    Plasmid

    #191691

    Purpose

    For knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    EN1 homology arms and p2a-mTagBFP2nls-3xHAtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-pTH-iCre-WPREpA

    Plasmid

    #213130

    Purpose

    Truncated rat TH promoter expressing iCre

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRK5-HA-GST-4EBP1-Low GC

    Plasmid

    #69018

    Purpose

    expresses tagged 4EBP1

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    EIF4EBP1 (EIF4EBP1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    lowGC content, wt is ~70% GC rich. Still, it code…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-FLAG-MTOR-L1460P

    Plasmid

    #69006

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-L1460P (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Leucine 1460 to Proline

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-FLAG-MTOR-C1483F

    Plasmid

    #69008

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-C1483F (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Cysteine 1483 to Phenylalanine

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pAAV-CAG-dLight1.1

    Plasmid

    #111067

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1 and AAV5

    Insert

    dLight1.1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

26. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

    Plasmid

    #196677

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    PKIα-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

    Plasmid

    #184632

    Purpose

    Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad67 (Gad1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBABE-HA-GOLPH3

    Plasmid

    #21687

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hsyn-GRAB_rDA-mut

    Plasmid

    #140558

    Purpose

    Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-dLight1.2

    Plasmid

    #111068

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1 and AAV5

    Insert

    dLight1.2
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-FLAG-MTOR-I2500F

    Plasmid

    #69014

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-I2500F (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Isoleucine 2500 to Phenylalanine

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-hSyn-dLight1.1

    Plasmid

    #111066

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1

    Insert

    dLight1.1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

33. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

    Plasmid

    #196678

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    PKIαmut-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-hsyn-GRAB_DA-mut

    Plasmid

    #140555

    Purpose

    Expresses the DA-insensitive control sensor GRAB_DA-mut in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    DA-insensitive sensor GRAB_DA-mut (control sensor)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-FLAG-MTOR-R2505P

    Plasmid

    #69015

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-R2505P (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Arginine 2505 to Proline

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-FLAG-MTOR-E1799K

    Plasmid

    #69009

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-E1799K (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Glutamate 1799 to Lysine

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-FLAG-MTOR-T1977R

    Plasmid

    #69011

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-T1977R (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Threonine 1977 to Arginine

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA3-FLAG-MTOR-F1888L

    Plasmid

    #69010

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-F1888L (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Phenylalanine 1888 to Leucine

    Promoter

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pAAV-Ef1a-DIO-VGAT-WPRE-pA

    Plasmid

    #39320

    Depositor

    Bernardo Sabatini

    Article

    Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV; R

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LRRK2-K1906M

    Plasmid

    #35946

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only