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Showing: 1601 - 1650 of 2321 results

  1. pQE30-TEV-pro-conA

    Plasmid
    #159527
    Purpose
    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.
    Depositor
    Joshua Mylne
    Article
    Nonis et al bioRxiv. 2020.
    Insert
    Canavalia ensiformis pro-concanavalin A
    Use
    Tags
    Six-Histidine tag and tobacco etch virus (TEV) pr…
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    159527-plasmid-map-sequence-id-317076

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  11. Halo-Erk2 donor

    Plasmid
    #113104
    Purpose
    Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence
    Depositor
    Janet Rossant
    Article
    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41
    Insert
    N terminal Erk2 donor region including homology arms and HaloTag
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113104-plasmid-map-sequence-id-217907

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  15. KG#45

    Plasmid
    #110926
    Purpose
    Vector for expressing proteins in most or all tissues, including nervous system, under control of the inducible heat shock promoter in C. elegans
    Depositor
    Kenneth Miller
    Article
    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1
    Inserts
    hsp-16-2 promoter
    unc-54 3' control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110926-plasmid-map-sequence-id-212564

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  25. pFTK090

    Plasmid
    #171362
    Purpose
    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    3'flanking region - penicillin gene cluster P. rubens
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Promoter
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    171362-plasmid-map-sequence-id-351793

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  26. pFTK089

    Plasmid
    #171361
    Purpose
    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    5'flanking region - penicillin gene cluster P. rubens
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Promoter
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    171361-plasmid-map-sequence-id-351792

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  29. Gli3ZF in AINGpCAGGS

    Plasmid
    #121972
    Purpose
    Gli3 zinc fingers
    Depositor
    James Briscoe
    Insert
    Gli3ZF (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus and C terminus deletion only nt 1411-1…
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121972-plasmid-map-sequence-id-241924

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  30. pDH5

    Plasmid
    #83775
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; his selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…
    Promoter
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83775-plasmid-map-sequence-id-159997

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  33. AAA

    Plasmid
    #78487
    Purpose
    2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Allan Jacobson
    Article
    Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d
    Insert
    CAN1-LUC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CAN1 contains no stop codon
    Promoter
    Available since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only

  37. pLSLZ.D

    Plasmid
    #51496
    Purpose
    pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    Zif268:FokI
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    SV40 NLS
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51496-plasmid-map-sequence-id-78596

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  41. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

    Plasmid
    #208625
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 72~197, other residues not included
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208625-plasmid-map-sequence-id-433638

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  42. pcDNA3.1-ARL16-His6

    Plasmid
    #216484
    Purpose
    Used for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tag
    Depositor
    Richard Kahn
    Insert
    ADP-ribosylation factor like 16 (ARL16 Human)
    Use
    Tags
    includes GSGSGSHHHHHH (His6) tag at C-terminus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216484-plasmid-map-sequence-id-426903

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Showing: 1601 - 1650 of 2321 results