We narrowed to 1,744 results for: lin tian

1. pSLIK-GFP

   Plasmid

   #66844

   Purpose

   Tet-inducible expression of GFP in mammalian cells

   Depositor

   Christian Metallo

   Article

   Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST exorh:mGreenLantern

   Plasmid

   #195988

   Purpose

   Transgenesis backbone

   Depositor

   Nicolas Rohner, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEM231 - [Pmlc-1 | gfp | cbr-tbb-2 UTR]

   Plasmid

   #159897

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | gfp | cbr-tbb-2 UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. JH02

   Plasmid

   #73511

   Purpose

   mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

   Depositor

   Bob Goldstein

   Article

   Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

   Insert

   pmex-5::mCherryo::PH::tbb-2
   

   Use

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   pmex-5

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCK029 exorh:EGFP

   Plasmid

   #195980

   Purpose

   Expression construct

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   exorh:EGFP
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTORKm43GWF

   Plasmid

   #112899

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTORKm43GWR

   Plasmid

   #112900

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of tdTomato.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p426-SNR52p-gRNA.csr-1.Y-SUP4t

   Plasmid

   #68060

   Purpose

   gRNA for csr-1 locus in N. crassa

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   gRNA for csr-1 locus
   

   Use

   CRISPR; N. crassa, fungi

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDN0604 (pDEST, HygR)

   Plasmid

   #210483

   Purpose

   pDEST vector with HygR marker for gene expression in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector for gateway…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDN0603 (pDEST, NatR)

    Plasmid

    #210482

    Purpose

    pDEST vector with NatR marker for gene expression in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR]

    Plasmid

    #159900

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | mcherry | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pmlc-1

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDYSCKO-ADE1

    Plasmid

    #120264

    Purpose

    pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    pDYSCKO cassette-ADE1 targeting guide
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    SNR52

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

    Plasmid

    #159896

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTORKm43GWC

    Plasmid

    #112898

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

    Type

    Empty backbone

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. attB-GFP

    Plasmid

    #65521

    Purpose

    Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-GFP
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR1A_no_ccDB_mUty

    Plasmid

    #158570

    Purpose

    V5-tagged sequence of mUty cloned into entry vector for recombination

    Depositor

    Christian Deschepper

    Article

    Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

    Insert

    Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTORKm43GWY

    Plasmid

    #112901

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

    Type

    Empty backbone

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR]

    Plasmid

    #159895

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]

    Plasmid

    #159898

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDYSCKO

    Plasmid

    #120263

    Purpose

    Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    DYSCKO cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    SNR52

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMNK55 - piRNAi 21ur-1224 - cku-80

    Plasmid

    #159820

    Purpose

    piRNA-induced RNA interference (piRNAi) to bias double-strand repair toward homologous recombination

    Depositor

    Christian Froekjaer-Jensen

    Article

    Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

    Insert

    cku-80 piRNAi 21ur-1224
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMNK54 - piRNAi 21ur-1224 - him-5

    Plasmid

    #159818

    Purpose

    piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

    Depositor

    Christian Froekjaer-Jensen

    Article

    Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

    Insert

    him-5 piRNAi 21ur-1224
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMNK56 - piRNAi 21ur-1224 - him-8

    Plasmid

    #159819

    Purpose

    piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

    Depositor

    Christian Froekjaer-Jensen

    Article

    Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

    Insert

    him-8 piRNAi 21ur-1224
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZF-BCB-GCN

    Plasmid

    #127476

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZF-BCB-PDZ

    Plasmid

    #127478

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-PDZ

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
    

    Use

    Cell-free transcription–translation

    Tags

    alpha 1-syntrophin PDZ domain

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pZF-ADD-L

    Plasmid

    #127477

    Purpose

    coding sequence for synthetic zinc finger ZF-ADD-L

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
    

    Use

    Cell-free transcription–translation

    Tags

    PDZ cognate peptide ligand (VKESLV)

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pZF-ADD-GCN

    Plasmid

    #127475

    Purpose

    coding sequence for synthetic zinc finger ZF-ADD-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDN0520 (pDEST, TRP1)

    Plasmid

    #210481

    Purpose

    pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Destination vector for gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTSK573

    Plasmid

    #190881

    Purpose

    For C-terminal -Halo Tagging of a gene of interest with TRP1 selection marker in yeast S. cerevisiae

    Depositor

    Tatiana Karpova, Gunjan Mehta

    Article

    Podh et al STAR Protoc. 2022 Dec 16;3(4):101900. do

    Insert

    HaloTag
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSEM262 - pDESTR4-R3 mosTI hygroR target

    Plasmid

    #159840

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    pDESTR4-R3 mosTI hygroR target
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-Myc-AIM2

    Plasmid

    #73958

    Purpose

    Express c-Myc-tagged human AIM2 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

    Insert

    absent in melanoma 2 (AIM2) (AIM2 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCW57.1_SPTLC1_WT

    Plasmid

    #181920

    Purpose

    3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1

    Depositor

    Christian Metallo

    Article

    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

    Insert

    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
    

    Use

    Lentiviral; Mammalian expression, , doxycycline i…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGFP-hCB2R

    Plasmid

    #179322

    Purpose

    Express human cannabinoid 2 receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Tomašević et al J Biol Chem. 2024 Jun;300(6):107330. doi

    Insert

    human cannabinoid 2 receptor (CNR2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

    Plasmid

    #89693

    Purpose

    Luciferase reporter plasmid targeted at csr-1 locus

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gsy-1 promoter, luciferase
    

    Use

    Yeast episomal vector

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    gsy-1

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSEM318 - sgRNA mosTI unc-119 single-copy

    Plasmid

    #159822

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 single-copy
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSEM319 - array co-injection fragment mosTI unc-119

    Plasmid

    #159823

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI unc-119
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSEM246 - MCS mosTI unc-119 target

    Plasmid

    #159821

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI unc-119 target
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCB24 pDEST crybb1:TagBFP

    Plasmid

    #195987

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann, Markus Affolter

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCB59 pDEST crybb1:mKate2

    Plasmid

    #195986

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann, Markus Affolter

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-Myc-ASC

    Plasmid

    #73952

    Purpose

    Express c-Myc-tagged human ASC in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

    Insert

    PYD and CARD domain containing, PYCARD (PYCARD Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET-21a(+)-AK1-His

    Plasmid

    #118977

    Purpose

    Bacterial expression plasmid for Adenylate kinase isoenzyme 1

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. p415-PtrpC-Cas9-TtrpC-CYC1t

    Plasmid

    #68059

    Purpose

    Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    humanized Cas9
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

    Plasmid

    #182353

    Purpose

    GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCMV-pro-IL1b

    Plasmid

    #73953

    Purpose

    Express murine pro-interleukin-1 beta in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

    Insert

    interleukin-1 beta (Il1b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. mVenus-IQ6 WT

    Plasmid

    #198201

    Purpose

    FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    MYO5A IQ domain (Myo5a Mouse)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pSEM320 - sgRNA mosTI unc-119 array insertion

    Plasmid

    #159824

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 array insertion
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

    Plasmid

    #182344

    Purpose

    sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Spacer 4
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCK082 desmaMCS:minprommCherry,exorh:mCerulean

    Plasmid

    #200015

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pDestattB_ubi:EGFP (pCM272)

    Plasmid

    #68339

    Purpose

    Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

    Depositor

    Leonard Zon, Christian Mosimann

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    EGFP
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    ubi

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

    Plasmid

    #182352

    Purpose

    Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge