We narrowed to 1,093 results for: Amot;

1. CMV-GEM-GECO1

   Plasmid

   #32442

   Purpose

   Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. px335-sgRNA-EF(Cas9n)-Dnmt3b-R

   Plasmid

   #122333

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. px335-sgRNA-EF(Cas9n)-Dnmt3b-L

   Plasmid

   #122334

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB[vasa-Cas9 PUb-ZsGreen]

   Plasmid

   #169012

   Purpose

   For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   Cas9
   

   Use

   Piggybac

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila melanogaster vasa

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Target-AIDmax

   Plasmid

   #139107

   Purpose

   Mammalian Target-AIDmax expression vector (pSI-626)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCXLE-Fbx15-cont2

   Plasmid

   #27081

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   FBXO15 (FBXO15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLR5-CAG-44B12mut23(Ser5ph)-SNAP

   Plasmid

   #174877

   Purpose

   PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   RNAPII Ser5ph mintbody-SNAP
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV2-SynTetOff-GFP

   Plasmid

   #190231

   Purpose

   AAV vector, high-level expression of EGFP in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK395 (AAVS1 CMV-OsTIR1(F74A))

   Plasmid

   #159992

   Purpose

   Expression of OsTIR1(F74A) in human cells

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CMV-OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-N2-Frankenbody-HA

    Plasmid

    #175292

    Purpose

    Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-HA frankenbody fused with mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Tet-OsTIR1(WT)-V5

    Plasmid

    #158663

    Purpose

    Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(WT) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pECE-HA-AMPKbeta1 wt

    Plasmid

    #31666

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)
    

    Use

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IB-mRuby3-FTH1

    Plasmid

    #192804

    Purpose

    Stable expression of mRuby3-FTH1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

    Plasmid

    #177265

    Purpose

    STREAMING-tag cassette

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag cassette
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174883

    Purpose

    Targeting vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Pou5f1
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTorPE-R-GECO1

    Plasmid

    #32465

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Lab constructed

    Tags

    6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. NLS-dCas9-trCIB1

    Plasmid

    #64119

    Purpose

    Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    dCas9-trCIB1 fusion
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    dCas9 (D10A H840A, catalytically inactive), trCIB…

    Promoter

    CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174882

    Purpose

    Targeting vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Nanog
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK427 (OsTIR1(WT) mAID-EGFP-Nluc)

    Plasmid

    #140658

    Purpose

    OsTIR1(WT)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(WT) mAID-EGFP-Nluc TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mDlx-SynTagMA_post-2A-mCerulean

    Plasmid

    #130899

    Purpose

    Synaptic Tag for Mapping Activity in GABAergic neurons

    Depositor

    Simon Wiegert

    Article

    Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

    Insert

    SynTagMA
    

    Use

    AAV

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    mDlx

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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