We narrowed to 670 results for: germes
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Plasmid#122299Purposeexpresses a part of RPS14 protein with a flag tagDepositorInsertRPS14 (RPS14 Human)
TagsFlagExpressionMammalianMutationdeleted amino acids 1 to 88PromoterCMVAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RPS14(2-88)
Plasmid#122298Purposeexpresses a part of RPS14 protein with a flag tagDepositorInsertRPS14 (partial) (RPS14 Human)
TagsFlagExpressionMammalianMutationdeleted amino acids 89 to 151PromoterCMVAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAK
Plasmid#23624DepositorInsertMAK (MAK Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIK86
Plasmid#47933PurposeC. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.DepositorInsertCas9
UseCRISPR and UnspecifiedPromoterT7Available SinceOct. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMLS328
Plasmid#73717PurposeC. elegans germline CRE expression vectorDepositorInsert2xNLS-CRE
UseCre/LoxExpressionWormPromoterPeft-3Available SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
PJ14
Plasmid#25912DepositorInsertIg germline mu switch region
ExpressionBacterialAvailable SinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
p64-101
Plasmid#25911DepositorInsertIg germline gamma-1 switch region alpha
ExpressionBacterialAvailable SinceAug. 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBS-gH4
Plasmid#45081DepositorInsertgerminal Histone H4
UseRNAi; In vitro transcriptionPromoterT7 sense, T3 antisenseAvailable SinceMay 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDD104 (Peft-3::Cre)
Plasmid#47551PurposeCre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germlineDepositorInsertCre Recombinase
UseCre/LoxExpressionWormPromotereef-1A.1 (eft-3)Available SinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDmVasa
Plasmid#165783PurposeDrosohphila vasa germline promoter and 5'UTRDepositorInsertVasa promoter
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-CR7T-gRNA2.1-nlsBFP
Plasmid#170515PurposegRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterCR7TAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
SP6-hCas9-Ce-mRNA
Plasmid#47911PurposeIn vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.DepositorInserthCas9 with C. elegans 5' and 3' UTRs
UseCRISPRPromoterSP6Available SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR3/ncd-gfp #10
Plasmid#66082Purposestable germline transformation of Drosophila to produce Ncd-GFP expressionDepositorInsertNcd kinesin-14 motor protein (ncd Fly)
TagsGFPExpressionInsectMutationD699LPromoterncd promoter (3.1kb upstream region of ncd start …Available SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHsp83-NLS-HA-2xMCP-2xTagRFP-T
Plasmid#71242PurposeUsed to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.DepositorInsertNLS-2xMCP-2xTagRFP-T
UseP element-mediated germline transformationTagsTagRFP-TExpressionInsectPromoterHsp83Available SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp-GBP-Tbro
Plasmid#179995PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.DepositorInsertGBP nanobody
TagsTrboExpressionInsectPromoterUAS-Gal4Available SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHsp83-NLS-HA-2xPCP-2xGFP
Plasmid#71243PurposeUsed to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.DepositorInsert2xPCP-2xGFP
UseP element-mediated germline transformationTagsEGFPExpressionInsectPromoterHsp83Available SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB[U6-3 wex2 PUb-DsRed]
Plasmid#169010PurposeFor germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.DepositorInsertwhite ex2 sgRNA
UsePiggybacExpressionInsectPromoterDrosophila melanogaster U6:3Available SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2
Plasmid#182351PurposeGermline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
ExpressionWormPromotereft-3Available SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only