We narrowed to 17,905 results for: RET

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pRExT2A-NT-PmNG

Plasmid

#232200

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMA3383

Plasmid

#46875

Purpose

A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1beta 3 (Gucy1b3 Rat)

Use

Retroviral

Promoter

LTR

Available Since

Aug. 13, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.CMV.SV40.THBS4-HA.SV40(polyA)

Plasmid

#155193

Purpose

Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-4 (Thbs4 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Kan-ADE2

Plasmid

#232105

Purpose

Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

GAL7

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag MKK6(K82A)

Plasmid

#13519

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6(K82A) (MAP2K6 Human)

Tags

Flag

Expression

Mammalian

Mutation

Lys-82Ala

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pQCXIH-tag RFPt- Rab11a WT

Plasmid

#161992

Purpose

Retroviral expression vector containing Rab11a tagged with RFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

RAB11A (RAB11A Canis Lupus)

Use

Retroviral

Tags

RFP

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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E42_gRunx1_gRNA3_dTet_NGFR

Plasmid

#189803

Purpose

Retroviral delivery of guide RNA against mouse Runx1

Depositor

Ellen Rothenberg

Article

Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

gRunx1_gRNA3 (Runx1 Mouse)

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGEL066

Plasmid

#124897

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

3xFlag

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgs2_B6

Plasmid

#170300

Purpose

A knock-out vector for the mouse Ptgs2 in C57BL6

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pQHA-USP11 CS puroR

Plasmid

#46750

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-GAL-Hyg

Plasmid

#232091

Purpose

Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

SpCas9 and Ec86 reverse transcriptase

Use

CRISPR

Expression

Yeast

Mutation

Ec86 reverse transcriptase is codon optimized for…

Promoter

GAL1-GAL10

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Sox17

Plasmid

#50781

Purpose

retroviral expression of mouse Sox17

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox17 (Sox17 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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2xFKBP-mCh-KDEL

Plasmid

#173010

Purpose

FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

2xFKBP-mCh-KDEL

Tags

mCherry

Expression

Mammalian

Promoter

Chicken beta actin (CAG)

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD-Myc

Plasmid

#201166

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRc/RSV Flag MKK3(glu)

Plasmid

#14670

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK3(glu) (MAP2K3 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser-189Glu Thr-193Glu

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pET-20b-M-Ntx-S1

Plasmid

#58716

Purpose

Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Tags

Methionine

Expression

Bacterial

Mutation

Changed Asparagine 1 to Serine

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Climp63(1–336)

Plasmid

#186957

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Plxna4-Fc-His

Plasmid

#72125

Purpose

Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna4 (Plxna4 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pPB CAG Otx2-IRES-EGFP

Plasmid

#153947

Purpose

piggyBac transposon vector with CAG promoter expressing Otx2 and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Otx2 (Otx2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-DEST-MatryoshCaMP6s

Plasmid

#100025

Purpose

Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Tags

V5 Epitope Tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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