162,717 results

1. HIP-flash (HOP-flash mutant)

   Plasmid

   #83466

   Purpose

   Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSP3545

   Plasmid

   #46888

   Purpose

   Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

   Depositor

   Gary Dunny

   Article

   Bryan et al Plasmid. 2000 Sep;44(2):183-90.

   Type

   Empty backbone

   Use

   Nisin-inducible expression; gram-positive bacteri…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PnisA

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPROEX Aqua

   Plasmid

   #42889

   Depositor

   Fabienne Merola

   Article

   Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

   Insert

   Aquamarine
   

   Use

   Tags

   6xHis and TEV cleavage

   Expression

   Bacterial

   Mutation

   T65S and H148G

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRBC-sfGFP wild type

   Plasmid

   #174075

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA-dCas9-p300 Core

   Plasmid

   #61357

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ATF4 1: Mouse ATF4 (CHOP11/cATF)-WT

   Plasmid

   #21845

   Depositor

   David Ron

   Insert

   ATF4 (Atf4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p-EGFP-C1-Flag-mFmr1(wt)

   Plasmid

   #87929

   Purpose

   Expresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.

   Depositor

   Stephanie Ceman

   Article

   Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

   Insert

   Fmr1 (Fmr1 Mouse)
   

   Use

   Tags

   Flag and eGFP

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RFP-LC3B

   Plasmid

   #200943

   Purpose

   RFP fused with LC3B for reporting LC3B lipidation

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-p53

   Plasmid

   #12091

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   72R variant of p53

   Promoter

   Available since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pnEA-vH_His-TEV-SEPT2_SEPT6

    Plasmid

    #174497

    Purpose

    bacterial co-expression of human SEPT2 and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge