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Showing: 6601 - 6620 of 40339 results

  1. pdCas9-M-3A2

    Plasmid
    #73435
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3A2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73435-plasmid-map-sequence-id-135340

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  4. pSico Dnmt1

    Plasmid
    #12165
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating Dnmt1 shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Insert
    Dnmt1 shRNA (Dnmt1 Mouse)
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Mutation
    EGFP is expressed from this plasmid as a marker, …
    Available Since
    June 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  5. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129028
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129028-plasmid-map-sequence-id-256131

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  8. pdCas9-M-4A6

    Plasmid
    #73434
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 4A6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73434-plasmid-map-sequence-id-135334

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  11. JA412

    Plasmid
    #49935
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD KL-1 (KLF4 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pdCas9-M-C4

    Plasmid
    #73427
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73427-plasmid-map-sequence-id-135327

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  15. Flag-M4-AR-W741C-S650A

    Plasmid
    #171252
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys-Ser650Ala mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171252-plasmid-map-sequence-id-336002

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  16. Flag-M4-AR-W741C-S650D

    Plasmid
    #171253
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys-Ser650Asp mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)
    Promoter
    CMV
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    171253-plasmid-map-sequence-id-335923

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  17. pdCas9-M-3F2

    Plasmid
    #73428
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73428-plasmid-map-sequence-id-135328

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  18. SL357

    Plasmid
    #49936
    Purpose
    Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1FL KL-1 (KLF4 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129036
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA8 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129036-plasmid-map-sequence-id-256165

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  20. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid
    #129053
    Purpose
    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129053-plasmid-map-sequence-id-256238

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Showing: 6601 - 6620 of 40339 results