We narrowed to 11,713 results for: NSI

1. pcDNA-FLAG-Co-NF-kB

   Plasmid

   #180197

   Purpose

   Expresses FLAG-tagged Capsaspora NF-kB protein in mammalian cells

   Depositor

   Thomas Gilmore

   Article

   Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

   Insert

   Co-NF-kB
   

   Tags

   FLAG-tagged fusion protein

   Expression

   Mammalian

   Mutation

   aa 2-1224

   Promoter

   CMV

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPQD1CB0768

   Plasmid

   #177058

   Purpose

   MoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPQD1CB0124

   Plasmid

   #177056

   Purpose

   MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPQD1CB0120

   Plasmid

   #177052

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   major latex protein-like (NmMLPL) from Nepeta mussinii
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ash1-5p

   Plasmid

   #169712

   Purpose

   Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed

   Depositor

   Tanja Mittag

   Article

   Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

   Insert

   Ash1 (ASH1 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Truncation including encoding amino acids 420-480…

   Promoter

   T7

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TAC-OKMS-KRAB-dCas9

   Plasmid

   #120356

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TAC-OK+9MS-KRAB-dCas9

   Plasmid

   #120357

   Purpose

   piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OK+9MS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [1-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. psiCHECK2 3'UTR KGA

   Plasmid

   #110402

   Purpose

   Luminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporter

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330.puro sgRNA KGA Stop1

   Plasmid

   #110405

   Purpose

   CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

    Plasmid

    #110408

    Purpose

    Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    CRISPR

    Promoter

    none

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Syn-FLEX-rc [ChromeT-GFP]

    Plasmid

    #153539

    Purpose

    AAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc-­His-­zfKitlga

    Plasmid

    #140292

    Purpose

    Express soluble His-tagged zebrafish Kitlga in insect cells

    Depositor

    Petr Bartunek

    Article

    Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

    Insert

    Kitlga (kitlga Zebrafish)
    

    Tags

    6x His

    Expression

    Insect

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGAL4-DBD-OLIG2wt-IDR

    Plasmid

    #145250

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and OLIG2wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    OLIG2wt-IDR (OLIG2 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGAL4-DBD-MNX1wt-IDR

    Plasmid

    #145248

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and MNX1wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    MNX1wt-IDR (MNX1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. RA35

    Plasmid

    #141126

    Purpose

    Expresses dimeric LacI repressor (LacI-11) under Rhl promoter

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

    Inserts

    dimeric LacI (LacI-11) (lacI Synthetic)
    AiiA (AQ980_RS12585 )
    

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    truncated/dimeric version of LacI (missing last 1…

    Promoter

    engineered rhl promoter (responds to C4-HSL) and …

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETDUET-GST AO7RE Y165A

    Plasmid

    #139428

    Purpose

    GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed tyrosine 165 to an alanine

    Promoter

    T7 promoter-1

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDUET-GST AO7RE P199A

    Plasmid

    #139427

    Purpose

    GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed proline 199 to an alanine

    Promoter

    T7 promoter-1

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETDUET-GST AO7RE I137A

    Plasmid

    #139319

    Purpose

    GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed isoleucine 137 to an alanine

    Promoter

    T7 promoter-1

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJA105

    Plasmid

    #128521

    Purpose

    Expresses amyloid human Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme T70N (LYZ Human)
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PCRII apoA-Ib

    Plasmid

    #121896

    Purpose

    Plasmid for making zebrafish apoA-Ib in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoA1b (apoa1b Zebrafish)
    

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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