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We narrowed to 8,284 results for: crispr grnas

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Showing: 6601 - 6620 of 8284 results

  1. pYUU

    Plasmid
    #159751
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159751-plasmid-map-sequence-id-313821

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  2. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid
    #75400
    Purpose
    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    hCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    35S
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75400-plasmid-map-sequence-id-146044

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  3. mU6-sgSik1_2nd-hU6-sgNT

    Plasmid
    #177213
    Purpose
    Expresses a Sik1-targeting (mU6) and a non-targeting (hU6) gRNAs and Cre recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_2nd/sgNT1
    Use
    Lentiviral
    Promoter
    mU6/hU6
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177213-plasmid-map-sequence-id-349895

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  4. mU6-sgSik1_1st-hU6-sgNT

    Plasmid
    #177212
    Purpose
    Expresses a Sik1-targeting (mU6) and non-targeting (hU6) gRNAs, and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgNT1
    Use
    Lentiviral
    Promoter
    mU6/hU6
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177212-plasmid-map-sequence-id-349892

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  5. Ccas9LacZ

    Plasmid
    #113083
    Purpose
    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Worm
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113083-plasmid-map-sequence-id-218959

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  12. SaCas9v3-Puro

    Plasmid
    #178813
    Purpose
    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178813-plasmid-map-sequence-id-354841

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  13. pBFC0687

    Plasmid
    #186692
    Purpose
    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_2xBsaI_NT (Guide Stuffer) gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186692-plasmid-map-sequence-id-371384

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  15. pSC38

    Plasmid
    #104812
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from AtUBQ10 promoter.
    Depositor
    Nevin Young
    Insert
    Medtr3g107390
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    104812-plasmid-map-sequence-id-202765

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  18. GFP-itis pBE-t

    Plasmid
    #195342
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V
    Depositor
    Alexis Komor
    Article
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA ctctacgcgggtcttgtagt
    Use
    CRISPR
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195342-plasmid-map-sequence-id-383223

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  19. bU6-sgNeo1-mU6-sgLkb1_2nd-hU6-sgNT

    Plasmid
    #177229
    Purpose
    Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgLkb1_2nd/sgNT1
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177229-plasmid-map-sequence-id-351963

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  20. bU6-sgLkb1_1st-mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177225
    Purpose
    Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_1st/sgNeo1/sgNT1
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177225-plasmid-map-sequence-id-351956

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Showing: 6601 - 6620 of 8284 results