We narrowed to 7,068 results for: poly
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Plasmid#145146PurposeMammalian expression plasmid for J chain of polymeric antibody classesDepositorInsertimmunoglobulin J chain (JCHAIN Human)
ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2A_mCherry_SV40pA (JDW 967)
Plasmid#224528PurposeA Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyADepositorInsertH2A-mCherry-SV40pA
UseGateway CloningAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.PHF19
Plasmid#125168PurposeExpresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EB3mCherry_cmv-LifeActGFP
Plasmid#133927PurposeLive dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeletonDepositorInsertEB3-mCherry and Lifeact-GFP (MAPRE3 Human)
ExpressionMammalianAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
IMPT-10314:GABBR2
Plasmid#157851PurposeHA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expressionDepositorAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
scADGFP
Plasmid#114434PurposeEpilepsy promoter enhanced with Arc elements driving GFP with SV40E polyADepositorInsertGreen fluorescent protein
UseAAVTagsER export sequence FCYENEVExpressionMammalianPromoterNovel synthetic activity-dependent promoterAvailable SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_ABE8e_IVT
Plasmid#201676PurposePlasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.DepositorInsertecTadA(8e)-nSpCas9
UseCRISPR; Vector for in-vitro transcriptionPromoterT3 promoterAvailable SinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQ79
Plasmid#175064Purposeexpresses mutant of the huntingtin exon1 in mammalian cellsDepositorInsertpolyQ79 (HTT Human)
ExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRR.1
Plasmid#69995PurposeMammalian expression of cytochrome p450 CYP2B6DepositorAvailable SinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2
Plasmid#85208PurposeBackbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expressionDepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianPromoterRNA polymerase III promoter for human U6 snRNA fo…Available SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro-Flag-Pol b
Plasmid#23257DepositorAvailable SinceMay 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
Tags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf
Plasmid#130976PurposeExpression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.DepositorAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1CCtoSS
Plasmid#89157PurposeRecombinant expression of PTBP1 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
Tagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC+] (AVA2626)
Plasmid#85444PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB PUb-integrase
Plasmid#183966PurposepiggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent markerDepositorInsertPhiC31 integrase
UsePiggybac insect transgenesis destination vectorPromoterAedes aegypti PolyubiquitinAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC+] (AVA2600)
Plasmid#85446PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCsy_complex
Plasmid#89232PurposePlasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage siteDepositorInsertsCsy1
Csy2
Csy3
Csy4
Tags6xHis-TEV proteaseAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
M2e-noro-VLP
Plasmid#201192PurposeExpresses noro-VLP decorated with influenza M2e in insect cellsDepositorInsertM2e-noro-VLP
TagsInfluenza M2e peptideExpressionInsectPromoterPolyhedrinAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
498 pSG5L HA RB del663 (NAAIRS)
Plasmid#10728DepositorInsertRB del663 (RB1 Human)
TagsHAExpressionMammalianMutationresidues 664 to 669 were replaced by NAAIRS, a fl…Available SinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only