165,567 results

1. pEHlyA5

   Plasmid

   #168298

   Purpose

   Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

   Depositor

   Luis Ángel Fernández

   Article

   Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

   Insert

   N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
   

   Tags

   E-tag, HA-tag, His-tag, Prescission site, and VHH…

   Expression

   Bacterial

   Promoter

   LacI-Plac

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFBP_2xNbLYS1::LUZ

   Plasmid

   #189918

   Purpose

   Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.

   Depositor

   Adam Steinbrenner

   Article

   Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   Various

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p426-SNR52p-gRNA.CAN1.Y-SUP4t

   Plasmid

   #43803

   Purpose

   Encodes a gRNA that targets Can1.Y in yeast.

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   CAN1.y gRNA (CAN1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   March 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

   Plasmid

   #205161

   Purpose

   express β-ACTIN-3′UTR-24xMS2

   Depositor

   Weirui Ma

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   β-ACTIN-3′UTR-24xMS2
   

   Tags

   tagBFP

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Rab7a-7

   Plasmid

   #55127

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab7a (RAB7A Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   N117S in NM_004637.5

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCVpuro-Cre

   Plasmid

   #34564

   Purpose

   Retroviral expression of Cre

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

   Insert

   Cre
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Promoter

   5'LTR

   Available Since

   Jan. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR CD19-FcGamma CAR

   Plasmid

   #113014

   Purpose

   for production of lentivirus encoding the CD19 CAR-Pfcgamma

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   CD19 CAR-P intracellular GFP (CD19 Human, Mouse, Synthetic)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

   Plasmid

   #225242

   Purpose

   Mammalian expression of iPASnFR-L in the cytosol

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-SopB

   Plasmid

   #183657

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX307-CRBN-3F-BirA

    Plasmid

    #220146

    Purpose

    Expresses CRBN-BirA for CRBN substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-ExRai-CKAR

    Plasmid

    #118409

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmGFP-P2A-K(AAA)20-P2A-RFP

    Plasmid

    #105688

    Purpose

    Double fluorescent stalling reporter K(AAA)20

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K(AAA)20
    P2A-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-RT001-LVB

    Plasmid

    #163381

    Purpose

    Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEEGFRtm-BFP (LVB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_Cas9–superNLS

    Plasmid

    #232430

    Purpose

    Expression plasmid for the SpCas9 nuclease with superNLS

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    Cas9–superNLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. ABE8e-NG

    Plasmid

    #226856

    Purpose

    Expresses ABE8e-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8e-NG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgCtr- LentiCRISPRv2

    Plasmid

    #107402

    Purpose

    Lentiviral expression of Cas9 and a control gRNA

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    control gRNA
    

    Use

    Lentiviral

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30-2-GPD1

    Plasmid

    #83911

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Mammalian expression: Z-lock cofilin dark state mutant

    Plasmid

    #141136

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Expression

    Mammalian

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCM184

    Plasmid

    #46012

    Depositor

    Mary Lidstrom

    Article

    Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

    Type

    Empty backbone

    Use

    Cre/Lox

    Promoter

    na

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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