164,826 results

1. pFlag-CMV1-hMD2

   Plasmid

   #13028

   Depositor

   Doug Golenbock

   Insert

   MD2 (LY96 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. hBax C3-EGFP

   Plasmid

   #19741

   Depositor

   Richard Youle

   Article

   Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

   Insert

   Bax (BAX Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHybrid-Electra2

   Plasmid

   #179475

   Purpose

   Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Inserts

   Electra2
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGal4AD-CIB1

   Plasmid

   #28245

   Purpose

   Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   Gal4AD (CIB1, GAL4 Budding Yeast)
   

   Use

   Tags

   CIB1

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CMV-mito-Citron1

   Plasmid

   #134305

   Purpose

   mitochondria-targeted direct-response citrate sensor

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citron1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pECE-M2-BAIAP2 wt

   Plasmid

   #31656

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   BAI1-associated protein 2 (BAIAP2 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pE-SUMO CRYAA

   Plasmid

   #80756

   Purpose

   Expression of human alpha A-crystallin in E. coli

   Depositor

   Larry David

   Insert

   CRYAA (CRYAA Human)
   

   Use

   Tags

   6xHIS and Smt3 SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR-nlsABACUS2-400n

   Plasmid

   #203726

   Purpose

   Gateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid

   Depositor

   Alexander Jones

   Article

   Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

   Insert

   nlsABACUS2-400n
   

   Use

   Synthetic Biology

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMK349 (BSD-P2A-mAID-mCherry)

   Plasmid

   #121183

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   BSD-P2A-mAID-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. iNos-eGFP

    Plasmid

    #207251

    Purpose

    Reporter for expression of eGFP under control of iNos promoter

    Depositor

    Michelle Farkas

    Article

    Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi:

    Insert

    iNos Promoter (Nos2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. human syndecan 2 wt

    Plasmid

    #64970

    Purpose

    Constitutive expression in mammalian cells

    Depositor

    Enric Espel

    Article

    Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

    Insert

    syndecan 2 (SDC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pUC18T-mini-Tn7T-Gm-eyfp

    Plasmid

    #65031

    Purpose

    GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    eyfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid

    #176836

    Purpose

    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    See Depositor Comments Below

    Promoter

    Tet operator (tetracycline inducible expression)

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBRC_double_attB_GFP_donor

    Plasmid

    #169692

    Purpose

    cargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion)

    Depositor

    John Wang

    Article

    Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-FLEX-GFP

    Plasmid

    #28304

    Depositor

    Edward Boyden

    Insert

    GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBXNH3CA_MBP

    Plasmid

    #132700

    Purpose

    expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Maltose binding Protein
    

    Use

    Tags

    Avi and His

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ES-FUCCI

    Plasmid

    #62451

    Purpose

    SMX_DEST vector with 3 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
    minPGK::Cherry-hCdt1(30-120)-bGHpA
    PGK::HygroR-bGpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_T7-ARG1

    Plasmid

    #106473

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid

    #172318

    Purpose

    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST17-UnaG

    Plasmid

    #203485

    Purpose

    For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMK231 (AAVS1 CMV-MCS-PURO)

    Plasmid

    #105924

    Purpose

    Donor for integration at the human AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLVX-Puro-TDP-43-Q331K

    Plasmid

    #133761

    Purpose

    Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed glutamine 331 to lysine.

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCK123 pDESTattB_exorh:mCerulean

    Plasmid

    #213512

    Purpose

    attB-containing pDEST plasmid, for use in Multisite Gateway reaction

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL4-ERSE1-luc2P-Hygro

    Plasmid

    #101789

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xERSE-I
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-TRE-GFP-Blast

    Plasmid

    #135673

    Purpose

    Dox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selection

    Depositor

    Florian Karreth

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CSII-IDR2-chimpTRIM5-FOS

    Plasmid

    #79065

    Purpose

    Express chimpanzee TRIM5alpha in mammalan cells

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5alpha(chimpanzee)-FOS
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

    Plasmid

    #187356

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a(+)_beta2M (MHC Class I light)

    Plasmid

    #187357

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    beta2M (MHC Class I light)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-N-RAM-d2tTA-TRE-mKate2

    Plasmid

    #140275

    Purpose

    N-RAM, the Npas4-dependent reporter

    Depositor

    Yingxi Lin

    Article

    Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

    Insert

    mKate2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

    Plasmid

    #185713

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed W403 and F753 to A

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge