We narrowed to 11,713 results for: NSI

1. PCRII apoBb.2

   Plasmid

   #121899

   Purpose

   Plasmid for making zebrafish apoBb.2 in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoBb.2 (apobb.2 Zebrafish)
   

   Use

   Unspecified

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCRII apoA-IVb.3

   Plasmid

   #121905

   Purpose

   Plasmid for making zebrafish apoA-IVb.3 in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   apoa4b.3 (apoa4b.3 Zebrafish)
   

   Use

   Unspecified

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-ChromeT-GFP

   Plasmid

   #123316

   Purpose

   AAV-mediated expression of ChromeT-GFP under the Syn promoter.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeT-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

   Promoter

   Syn

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCRII apoA-IVa

   Plasmid

   #121902

   Purpose

   Plasmid for making zebrafish apoA-IVa in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoA4a (apoa4a Zebrafish)
   

   Use

   Unspecified

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCRII apoA-Ia

   Plasmid

   #121895

   Purpose

   Plasmid for making zebrafish apoA-Ia in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   apoA-Ia (apoa1a Zebrafish)
   

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a_6HT-TBP6.7(P46A)

   Plasmid

   #112721

   Purpose

   This mutation (P46A) marks the first residue within the evolved region of U1A.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P46A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P46A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX6P-1 BICC FL

    Plasmid

    #112998

    Purpose

    For protein expression and purification of full-length Drosophila BicC

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length BicC (BicC Fly)
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-30a(+)-N-SH2

    Plasmid

    #111274

    Purpose

    express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    

    Tags

    HHHHHHENLYFQG

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPR sgAMDHD1

    Plasmid

    #102316

    Purpose

    genetic depletion of AMDHD1

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgAMDHD1 (AMDHD1 Human)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAC_N_TBG1

    Plasmid

    #111696

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    TBG1 (TUBG1 Human)
    

    Expression

    Mammalian

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAC_C_COX14

    Plasmid

    #111663

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    COX14 (C12orf62 Human)
    

    Expression

    Mammalian

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAC_C_AKIP

    Plasmid

    #111660

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    AKIP (AURKAIP1 Human)
    

    Expression

    Mammalian

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAC_C_MGST3

    Plasmid

    #111670

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    MGST3 (MGST3 Human)
    

    Expression

    Mammalian

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR3-vsv-CenpB 1-166

    Plasmid

    #108474

    Purpose

    expression of vsv-CenpB 1-166

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    CenpB 1-166 AA (CENPB Human)
    

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    encodes only the DNA-binding domain of CENP-B

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR3-vsv-Mis12-dCEN-Incenp

    Plasmid

    #108477

    Purpose

    expression of vsv-Mis12-dCEN-INCENP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    Mis12-dCEN-INCENP (INCENP Human)
    

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    CEN box replaced by Mis12

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_BZRAP1-2/3

    Plasmid

    #91551

    Purpose

    Protein expression and purification of human SH3 domain construct BZRAP1-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BZRAP1-2/3 (TSPOAP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_SH3RF2-2/3

    Plasmid

    #91557

    Purpose

    Protein expression and purification of human SH3 domain construct SH3RF2-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3RF2-2/3 (SH3RF2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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