We narrowed to 9,184 results for: Pol;

1. pcDNA5-FRT-TO-EGFR*-CUbo(K63R)

   Plasmid

   #212822

   Purpose

   Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   EGFR* (EGFR Budding Yeast, Human)
   

   Tags

   CUbo(K63R)

   Expression

   Mammalian

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-DDK_S172A_M173A_V195A RAD23A

   Plasmid

   #201576

   Purpose

   Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutations S172A, M173A and V195A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-DDK_WT RAD23A_aa 111-363 (delta UBL domain)

   Plasmid

   #201460

   Purpose

   Expresses a mutant form of human RAD23A that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks N-terminal residues 1-110

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6-AN-DDK_WT RAD23A_delta aa 161-201 (delta UBA1 domain)

   Plasmid

   #201463

   Purpose

   Expresses a mutant form of human RAD23A that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 161-201

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-AN-DDK_WT aa 1-317 RAD23A (delta UBA2 domain)

   Plasmid

   #201465

   Purpose

   Expresses a mutant form of human RAD23A that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks C-terminal residues 318-363

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV6-AN-DDK_WT RAD23A_delta aa 111-136

   Plasmid

   #201466

   Purpose

   Expresses a mutant form of human RAD23A lacking residues 111-136. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 111-136

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV6-AN-DDK_WT RAD23A_delta aa 137-160

   Plasmid

   #201467

   Purpose

   Expresses a mutant form of human RAD23A lacking residues 137-160. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 137-160

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV6-AN-DDK_WT RAD23A_delta aa 202-230

   Plasmid

   #201468

   Purpose

   Expresses a mutant form of human RAD23A lacking residues 202-230. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 202-230

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV6-AN-DDK_WT RAD23A_delta aa 287-316

   Plasmid

   #201469

   Purpose

   Expresses a mutant form of human RAD23A lacking residues 287-316. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 287-316

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

    Plasmid

    #196878

    Purpose

    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-N-gTBD
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196881

    Purpose

    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

    Plasmid

    #191331

    Purpose

    Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
    

    Expression

    Mammalian

    Mutation

    DYNC1H1 motor domain aa 1453-4644

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV.CBA.YFP.miR-E_shIrak1-2

    Plasmid

    #180398

    Purpose

    Producing AAV that encodes mouse Irak1 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shIrak1-2 (Irak1 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV.CBA.YFP.miR-E_shIrak1-3

    Plasmid

    #180399

    Purpose

    Producing AAV that encodes mouse Irak1 shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shIrak1-3 (Irak1 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV.CBA.YFP.miR-E_shIrak1-1

    Plasmid

    #180397

    Purpose

    Producing AAV that encodes mouse Irak1 shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shIrak1-1 (Irak1 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA528 CHMP1B 4-199

    Plasmid

    #115325

    Purpose

    Expresses residues 4-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-23

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX2T-TEV CHMP1B 4-178

    Plasmid

    #108284

    Purpose

    Expresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 179-199

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28_6xHis-PARP1delZn1-L713F

    Plasmid

    #173937

    Purpose

    Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn1-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 1-96; L713F, V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28_6xHis-PARP1-G871L

    Plasmid

    #174798

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G871L (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G871L, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28_6xHis-PARP1-A870L

    Plasmid

    #174797

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A870L (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A870L, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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