174,131 results

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pCAG_mRuby2_smFP FLAG

Plasmid

#59760

Purpose

encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-CMV-14-WDR5

Plasmid

#59974

Purpose

Mammalian Expression of WDR5-FLAG

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 (WDR5 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-CIB1-mCerulean-MP

Plasmid

#58366

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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p201G Cas9

Plasmid

#59178

Purpose

Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
sGFP

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-Nesprin3-C-18

Plasmid

#54203

Purpose

Localization: Nuclear Membrane , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Nesprin3 (SYNE3 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSUMO2

Plasmid

#52259

Purpose

Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBT378_pattB-pCA-GFP-pA-attB

Plasmid

#52554

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

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pEF-Bos-FLAG RIG-I MIII

Plasmid

#52877

Purpose

Expresses RIG-I with a mutation to motif III in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

DDX58 (RIGI Human)

Tags

FLAG

Expression

Mammalian

Mutation

T409A, S411A

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pVITRO1-102.1F10-IgG1/λ

Plasmid

#50366

Purpose

Expresses grass pollen allergen Phl p 7 specific human IgG1/λ antibody isotype (102.1F10-IgG1/λ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

grass pollen allergen Phl p 7 specific human Gamma 1 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette

Expression

Mammalian

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJFA344C7

Plasmid

#49236

Purpose

TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

TALE-TET1CD Backbone (TET1 Human, Synthetic)

Use

TALEN; Tale-tet1 expression vector

Promoter

EF1a

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pICH41780

Plasmid

#48019

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pCEV-G1-Km

Plasmid

#46813

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pT7EGFPgRNA

Plasmid

#46760

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

egfp target

Use

CRISPR

Promoter

T7

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-rM3D(Gs)

Plasmid

#45549

Purpose

Expresses rM3D (Gs) neuronal activator

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

rM3D (Gs)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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CENP-B mCherry

Plasmid

#45219

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

CENP-B mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-CaURA3

Plasmid

#44872

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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Pet28a_Human_H2B.1

Plasmid

#42630

Depositor

Joe Landry

Insert

Human H2B.1 (H2BC8 Human)

Tags

Untagged

Expression

Bacterial

Promoter

T7

Available Since

Feb. 22, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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mRIP3 GFP wt

Plasmid

#41382

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pET11a-4SS-HEWL

Plasmid

#39233

Depositor

Harald Schwalbe

Article

Schlorb et al J Biomol NMR. 2005 Oct;33(2):95-104.

Insert

Hen egg white lysozyme (LYZ Chicken)

Expression

Bacterial

Mutation

N-terminal Met

Promoter

T7

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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