We narrowed to 13,271 results for: cDNA

1. pGFAP-TetOn3G

   Plasmid

   #133728

   Purpose

   Plasmid contains the TetOn3G-transactivator sequence under control of a GFAP promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   TetOn3G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pGFAP

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TagBFP(S2)2(S4)2-gs

   Plasmid

   #228072

   Purpose

   Mammalian expression of coiled coil segments (S2)x2-(S4)x2, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

   Depositor

   Roman Jerala

   Article

   Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

   Insert

   BFP, S2, S4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-MRGBP

   Plasmid

   #15325

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

   Insert

   MRG-binding protein (MRGBP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

4. BirA-Myc_N_TAL1

   Plasmid

   #167728

   Purpose

   Expresses TAL1 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   TAL1 (TAL1 Human)
   

   Use

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Shp2 biosensor

   Plasmid

   #134346

   Purpose

   FRET biosensor. To visualize Shp2 activity by FRET

   Depositor

   Yingxiao Wang

   Article

   Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

   Insert

   Shp2 Biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mito-mGarnet2

   Plasmid

   #104310

   Purpose

   codes for a fusion of fluorescent protein mGarnet2 and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

   Depositor

   Ulrich Nienhaus

   Article

   Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

   Insert

   mito-mGarnet2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HA-AMOT p130 Y242/287A

   Plasmid

   #32822

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

   Insert

   AMOT p130 (AMOT Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E5D, Y242/287A

   Promoter

   CMV

   Available Since

   Jan. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

8. FL-mCherry

   Plasmid

   #78862

   Purpose

   DULIP negative control (prey) to exclude unspecific background binding

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   mCherry
   

   Use

   Expression vector

   Tags

   Firefly luciferase, V5-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-sav

   Plasmid

   #32834

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   Sav (SAV1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

10. rASAP

    Plasmid

    #178205

    Purpose

    Ratiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.

    Depositor

    Stefan Heinemann

    Article

    Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

    Insert

    rASAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pActin-miRFP670nano

    Plasmid

    #127428

    Purpose

    Human beta-actin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano-human beta-actin
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. mNFAT4-462:TALE:VP64:KRɸ

    Plasmid

    #160662

    Purpose

    Constitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    mNFAT4-462:TALE:VP64:KRɸ
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. NLSx3-CRY2PHR-p65

    Plasmid

    #64124

    Purpose

    Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    CRY2PHR-p65
    

    Use

    Tags

    His, NLSx3, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. hMTP

    Plasmid

    #124281

    Purpose

    Expresses human MTP in mammalian cells.

    Depositor

    Mahmood Hussain

    Article

    Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

    Insert

    MTTP (MTTP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-Mob

    Plasmid

    #32835

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    Mob (MOB1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRPML1-CFP

    Plasmid

    #18827

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

17. 4xHRE_minCMV-sfGFP-CMV_dsRed Exp

    Plasmid

    #117401

    Purpose

    sfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hTPST1

    Plasmid

    #11252

    Depositor

    Hyeryun Choe

    Article

    Choe et al Cell. 2003 Jul 25. 114(2):161-70.

    Insert

    TPST1 (TPST1 Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

19. EGFP-Rubicon deltaCT

    Plasmid

    #28040

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    3xFlag and EGFP

    Expression

    Mammalian

    Mutation

    delta880-end

    Promoter

    Available Since

    March 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-Rubicon delta182

    Plasmid

    #28041

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    3xFlag and EGFP

    Expression

    Mammalian

    Mutation

    delta 1-182aa

    Promoter

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

21. Plasma membrane-targeted optodPLD

    Plasmid

    #140061

    Purpose

    Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    H170A (catalytic dead)

    Promoter

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFH2.30_Gag-MCP-dpol-T2A-eGFP

    Plasmid

    #205538

    Purpose

    Express HIV Gag-MCP-dpol-T2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    HIV Gag-MCP-dpol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    pol frameshift sequence recoded

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Mammalian_SpCas9_Target-AID2S

    Plasmid

    #188648

    Purpose

    Expresses SpCas9_Target-AID2S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID2S-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. M-NMn-VP64

    Plasmid

    #48676

    Purpose

    Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null

    Promoter

    CMV

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mApple-hCOL1A1

    Plasmid

    #140113

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 2xMyc-tdMCP-SNAP (tdMCP standard)

    Plasmid

    #226166

    Purpose

    For transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    2xMyc-tdMCP-SNAPtag
    

    Use

    Lentiviral

    Tags

    2xMyc-tdMCP and EGFP-IRES-BlastR

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCherry-2xCL-YFP-Vpr

    Plasmid

    #105215

    Purpose

    Bi-functional Fluorescent reporter protein to visualize HIV-1 fusion and core-trafficking

    Depositor

    Gregory Melikyan

    Article

    Sood et al J Biol Chem. 2017 Oct 18. pii: M117.8180

    Insert

    Vpr
    

    Use

    Tags

    PC2x (SQNY/IRKVL), eYFP, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNIR-FbGFPY58/C-PKI

    Plasmid

    #184685

    Purpose

    NIR-Fb-based PKA inhibitor

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbGFPY58/C-PKI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Mammalian_SpCas9_Target-AID3S

    Plasmid

    #188649

    Purpose

    Expresses SpCas9_Target-AID3S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID3S-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mVenus-hCOL1A1

    Plasmid

    #140111

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge