We narrowed to 37,082 results for: ELL

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PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

Plasmid

#110623

Purpose

It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH mScarlet-I- IRES- sYFP2 PH

Expression

Mammalian

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR1A-NTAP-C (w324-1)

Plasmid

#17404

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame C

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

CBP and SBP

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim1 (Bl/6)

Plasmid

#49205

Purpose

expresses Flag tagged mouse Tim1

Depositor

Lawrence Kane

Publication

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (BL/6 allele) (Havcr1 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
sgRNA 1 GAPDH

Plasmid

#83808

Purpose

Express Sg-1 sgRNA targeting GAPDH

Depositor

Bo Feng

Publication

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-hPhyBY276H-mCherry-HRasCT

Plasmid

#100540

Purpose

Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

Depositor

Kazuhiro Aoki

Publication

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)

Tags

mCherry fused with HRas C-terminus for plasma mem…

Expression

Mammalian

Mutation

Human codon optimized PhyB (1-908 aa), Y276H muta…

Promoter

CAG promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pQC membrane CFP IX

Plasmid

#37336

Depositor

Connie Cepko

Publication

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

membrane CFP

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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LAMP1-APEX2

Plasmid

#215555

Purpose

Stable, inducible expression in lysosome

Depositor

Nevan Krogan

Publication

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

LAMP1-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-6xHis-AviTag-ALFAtag-eGFP

Plasmid

#195854

Purpose

Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

Depositor

John Ngo

Publication

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

6xHis-AviTag-ALFAtag-eGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TEVp

Plasmid

#162599

Purpose

TEV protease under CMV promoter

Depositor

Masaharu Somiya

Publication

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

TEV protease

Expression

Mammalian

Promoter

CMV promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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3xHA-BioID_pAS28

Plasmid

#118223

Purpose

expresses 3xHA-tagged BioID in C. elegans intestine

Depositor

Jessica Feldman

Publication

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

BioID (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

R118G

Promoter

ges-1p

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Rab7(WT)

Plasmid

#112959

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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5.2 kB VEGF-luc

Plasmid

#27987

Depositor

Patricia D'Amore

Publication

Loureiro et al Biochem Biophys Res Commun. 2005 Jan 14.

Insert

5.2 kB VEGF

Use

Luciferase

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
3XFlag DVL2 (E499-G)

Plasmid

#24803

Depositor

Jeff Wrana

Publication

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

E499G

Available since

June 22, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
HIV-1-GFP ΔEnv

Plasmid

#217437

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

Depositor

Jarrod Johnson

Publication

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

Plasmid

#209727

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Publication

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95K.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV-VSVgSS-15F11-TM

Plasmid

#207317

Purpose

Functional incorporation of 15F11 scFv (anti-HA) into envelope of virions

Depositor

Feng Zhang

Publication

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

15F11 ScFv

Expression

Mammalian

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_SFV_E1E2

Plasmid

#207308

Purpose

Mammalian expression of Semliki Forest virus E1E2 protein

Depositor

Feng Zhang

Publication

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SFV_E1E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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PB-SRT-Puro

Plasmid

#154884

Purpose

piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Joseph Dougherty, Rob Mitra

Publication

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

piggyBac SRT

Use

Transposon

Expression

Mammalian

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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epB_UbC_tdMS2cp-tagRFP-Tx2

Plasmid

#119908

Purpose

ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

Depositor

Orion Weiner

Publication

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

HA-tdMS2cp-tagRFP-Tx2-nls

Expression

Mammalian

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-6xHis-(pos16)GFP

Plasmid

#89249

Purpose

Expresses GFP with +16 theoretical charge in E.Coli

Depositor

David Liu

Publication

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos16)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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