We narrowed to 67,877 results for: TOR

1. pDestTol2CG2-mnx1:ReaChR-citrine

   Plasmid

   #169046

   Purpose

   Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   ReaChR-Citrine
   

   Tags

   Citrine

   Expression

   Mammalian

   Promoter

   3xMnx1

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. P2RX1-bio-His

   Plasmid

   #53359

   Purpose

   Expresses full-length P2X purinoceptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   P2RX1 (P2RX1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. [pSK467] pLJC5 HA-Depdc5

   Plasmid

   #109343

   Purpose

   Lenti-viral expression of HA-tagged wildtype Depdc5

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVeBtA1

   Plasmid

   #238986

   Purpose

   Mammalian expression of bovine arrestin-1 with Venus N-terminal fusion

   Depositor

   Vsevolod Gurevich

   Article

   Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

   Insert

   arrestin-1 (SAG Bovine)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PM-FRB-CFP

   Plasmid

   #67517

   Purpose

   FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

   Insert

   mTOR (MTOR Human)
   

   Tags

   CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

   Expression

   Mammalian

   Mutation

   FRB domain only (residues 2019–2114)

   Promoter

   CMV

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-MyosinVa-Ctail

   Plasmid

   #110169

   Purpose

   Dominant negative effect by competing with MyosinVa C-terminal tail interactions

   Depositor

   Jose Esteban

   Article

   Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

   Insert

   Myosin Va (Myo5a Mouse)
   

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   amino acids 1444-1853

   Promoter

   CMV

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRVL-6

   Plasmid

   #104584

   Purpose

   Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
   

   Expression

   Mammalian

   Mutation

   L234A/L235E/G237A and K322A/P331S

   Promoter

   CMV

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SEO-CaMKII-TVA-P2A-N2cG

   Plasmid

   #172363

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinase

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PF4-bio-His

   Plasmid

   #53411

   Purpose

   Expresses full-length Platelet factor 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PF4 (PF4 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9 TadCBEa

    Plasmid

    #193782

    Purpose

    Express TadCBEa (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa-SpCas9D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBO.ACT006

    Plasmid

    #182716

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    GFP in cloning site of crRNA for easier screening…

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-CREB

    Plasmid

    #68550

    Purpose

    AAV expression of CREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    CREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-gp78c/2767

    Plasmid

    #11429

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78c (AMFR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-GNPTAB

    Plasmid

    #78108

    Purpose

    Mammalian expression of human GNPTAB with C terminal myc tag

    Depositor

    Thomas Braulke

    Article

    Franke et al J Biol Chem. 2013 Jan 11;288(2):1238-49.

    Insert

    GNPTAB (GNPTAB Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

15. p3xFLAG-CMV-Tlr4

    Plasmid

    #27148

    Depositor

    Bruce Beutler

    Article

    Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

    Insert

    toll-like receptor 4 (Tlr4 Mouse)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Native signal peptide sequence (original amino ac…

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA-D2s-L-Venus

    Plasmid

    #19966

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
    

    Tags

    Flag and monomeric Venus

    Expression

    Mammalian

    Available Since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-KG-Itch/2793

    Plasmid

    #11434

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    Itch (Itch Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-cT3G-cERH-ZNF281

    Plasmid

    #222923

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    ZNF281 (ZNF281 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC-HYG-YR

    Plasmid

    #61765

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Hygromycin
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Promoter

    trpC promoter

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9 TadCBEe

    Plasmid

    #193836

    Purpose

    Express TadCBEe (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDe-SpCas9D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti_CamKIIa_mKate2

    Plasmid

    #96942

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Oleksandr Shcheglovitov, Ricardo Dolmetsch

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    far-red fluorescent protein
    

    Use

    Lentiviral

    Mutation

    E87D (please see depositor comments below)

    Promoter

    CaMKIIa

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBS L30 Ruby3-Rab6A

    Plasmid

    #135653

    Purpose

    Rab6A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

    Insert

    Rab6A
    

    Tags

    Ruby3

    Expression

    Mammalian

    Promoter

    L30

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pHDR2_DAZL-T2A-mGreenLantern-PuroTK

    Plasmid

    #222917

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP.N1-HDAC6.delta Buz

    Plasmid

    #36190

    Depositor

    Tso-Pang Yao

    Article

    Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

    Insert

    HDAC6..delta Buz (HDAC6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the C-terminal ub binding domain (BUZ…

    Promoter

    CMV

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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25. p2T-cmv-SpCas9-TadDE-BlastR

    Plasmid

    #193850

    Purpose

    Express TadDE in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadDE
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. [pSK468] pLJC5 Flag-Depdc5

    Plasmid

    #109337

    Purpose

    Lenti-viral expression of Flag-tagged wildtype Depdc5

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLVX Flag RagC S75N

    Plasmid

    #112757

    Purpose

    express Flag-RagC (S75N mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (S75N) (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    S75N

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

    Plasmid

    #162335

    Purpose

    Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pTon1(acgfp1)

    Plasmid

    #233648

    Purpose

    Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

    Depositor

    Takeo Kubo

    Article

    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

    Insert

    target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    

    Use

    Amphibian expression, tet-on

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pPPEDs

    Plasmid

    #162469

    Purpose

    protoplast test vector of pPPED

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Use

    Prime editing destination vector for transient ex…

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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