159,141 results

1. jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)

   Plasmid

   #79785

   Purpose

   genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a barcode landing pad

   Depositor

   Anthony Zador

   Insert

   MAPP-nL
   

   Use

   Synthetic Biology; Sindbis virus genomic plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherry-RatLC3B-C-7

   Plasmid

   #55128

   Purpose

   Localization: Microtubule-Binding Protein, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   LC3B
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pSCD-Casp3-L-tev-S

   Plasmid

   #226230

   Purpose

   Express TEVP-cleavable caspase-3

   Depositor

   Michael Elowitz

   Article

   Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

   Insert

   Caspase-3 (CASP3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO-GAlphai1-RLuc8

   Plasmid

   #140973

   Purpose

   Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphai1-RLuc8 (GNAI1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTPGI_dCas9_VP64

   Plasmid

   #49013

   Purpose

   encodes yeast-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   pTPGI (galactose+aTc inducible)

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSynSRE-T-Luc

   Plasmid

   #60444

   Purpose

   Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

   Depositor

   Timothy Osborne

   Article

   Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

   Insert

   HMG-CoA synthase promoter (-324/-225)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMRX-IZ-ANXA1-mRuby3

   Plasmid

   #184888

   Purpose

   Stable expression of ANXA1-mRuby3 (Annexin A1-mRuby3) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

   Insert

   ANXA1 (ANXA1 Human)
   

   Use

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKDsgRNA-NT

   Plasmid

   #89960

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   empty gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-R-GECO1.2

   Plasmid

   #45494

   Purpose

   Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   R-GECO1.2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

   Promoter

   CMV

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTARA

    Plasmid

    #31491

    Depositor

    Kathleen Matthews

    Article

    Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

    Insert

    RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N823D

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBR322-TIMER

    Plasmid

    #103056

    Purpose

    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

    Depositor

    Dirk Bumann

    Article

    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

    Insert

    TIMER-bac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations: S4T and S179T

    Promoter

    constitutive ybaJp promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-Rac1-2G

    Plasmid

    #66111

    Purpose

    Second generation Rac1 FRET biosensor for lentivirus production

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Lentiviral

    Tags

    His-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZS47a luxI-laa RBS

    Plasmid

    #48892

    Purpose

    ArsR-regulated expression of luxI-laa

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    LuxI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pArsR

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMK003

    Plasmid

    #236570

    Purpose

    Cloning vector for PaqCI Golden Gate assembly of ISDra2 guides into ISDra2 plant expression vector

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV_BiLAMP5e3_Gq_dTomato

    Plasmid

    #213916

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MsACR1-mCerulean3-pAAV2-hSyn

    Plasmid

    #204965

    Purpose

    Panneuronal expression of MsACR1 fused to mCerulean3

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    MsACR1
    

    Use

    AAV

    Tags

    mCerulean3

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

    Plasmid

    #204970

    Purpose

    Soma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    MsACR1
    

    Use

    AAV

    Tags

    mCerulean3

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p8328 pLIX YAP1 S94A

    Plasmid

    #184529

    Purpose

    Expression of YAP1 S94A

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    YAP1 S94A (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S94A

    Promoter

    TRE promoter, Tet ON

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti PGK Blast V5-LUC (w528-1)

    Plasmid

    #19166

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDest-566

    Plasmid

    #11517

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge