169,691 results

Showing: 6681 - 6700 of 169691 results

Results per page:

Sort results by:

pLX-sgRNA

Plasmid

#50662

Purpose

Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-FIX(a) Emi HC

Plasmid

#113664

Purpose

For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FX(a) Emi HC and the Emi LC.

Depositor

Robert Peters

Article

Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

Insert

Emicizumab anti-FIX/FIXa heavy chain

Expression

Mammalian

Promoter

CMV Promoter

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hUbc13

Plasmid

#51131

Purpose

human ubc13, E2 for synthesis k63 linked ubiquitin chain

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

ubc13 (UBE2N Human)

Tags

His6 and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
gTBEv5

Plasmid

#220621

Purpose

vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

Insert

nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)

Expression

Mammalian

Mutation

hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

Promoter

hU6, EF1a

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-Grx1-RoTq-On

Plasmid

#234467

Purpose

Mammalian expression of Grx1-RoTq-On

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

Insert

Grx1-RoTq-On

Use

AAV

Expression

Mammalian

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TANGO1S-mScarlet-i

Plasmid

#165461

Purpose

Encodes TANGO1S tagged with mScarlet-i

Depositor

David Stephens

Article

McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi

Insert

TANGO1S

Tags

mScarlet-i

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSNAPf-Nup43

Plasmid

#98276

Purpose

mammalian expression of SNAPf-Nup43

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup43 (NUP43 Human)

Tags

SNAPf-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH-MYC

Plasmid

#101765

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal Myc-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

Myc

Expression

Mammalian

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_hSyn1_nLightG

Plasmid

#187179

Purpose

Expresses green norepinephrine indicator nLightG in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Has Service

AAV9

Insert

nLightG

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRE-Tn7-147

Plasmid

#118563

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Flag-ER-Hoxb8-IRES-GFP

Plasmid

#222292

Purpose

Less commonly used plasmid for generating ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

Mark Kamps, David Sykes

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DRD2-DuET

Plasmid

#213228

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

DRD2 (DRD2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAS

Plasmid

#206994

Purpose

Plasmid expressing both SpCas9 and SaCas9

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Insert

SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )

Tags

P2A

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDNR-gRNA

Plasmid

#206990

Purpose

A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Insert

Donor plasmid-targeting SaCas9-gRNA

Expression

Mammalian

Promoter

U6

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF525-EF1a-Hygro-P2A-mCherry-lenti

Plasmid

#115796

Purpose

Expresses mCherry in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mCherry

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF_LuxR

Plasmid

#140039

Purpose

Constutitive expression of LuxR protein.

Depositor

Yolanda Schaerli

Article

Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

Insert

pLuxL and LuxR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Tet-inducible Luciferase reporter

Plasmid

#64127

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

luciferase

Use

Luciferase

Expression

Mammalian

Promoter

TetOx13-CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYIC

Plasmid

#18673

Depositor

Han Htun

Article

Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

Type

Empty backbone

Tags

3Myc-EYFP-HA-His6 and ECFP-HA-His6

Expression

Mammalian

Available Since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212829

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

Depositor

Wolfgang Enard

Article

Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Promoter

CAG, TetOn

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge