19,658 results

1. phage-cmv-cfp-24xpp7

   Plasmid

   #40652

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   CFP-24xPP7
   

   Use

   Lentiviral

   Tags

   CFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-Myc-Parkin

   Plasmid

   #17612

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti X1 Puro empty (w610-1)

   Plasmid

   #20953

   Purpose

   3rd gen lentiviral empty control vector, Puro

   Depositor

   Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCMV-intron myc Rab2 S20N

   Plasmid

   #46780

   Purpose

   expression of S20N Rab2a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB2 (RAB2A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S20N dominant negative

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

5. TIGRE docking site targeting vector

   Plasmid

   #61580

   Purpose

   Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169192

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Unspecified

   Tags

   6His-TEV

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAT-PB-CD2APtk

   Plasmid

   #86004

   Purpose

   homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   DsRedEx_T2A_puro_dTK
   

   Use

   Piggybac exciseable

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

   Plasmid

   #104567

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
   

   Use

   CRISPR

   Tags

   NLS-3xHA-VPR

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINE GFP nanobody:Halo:His6

   Plasmid

   #111090

   Purpose

   Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

   Depositor

   Lennart Wirthmueller

   Article

   Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

   Insert

   GFP Nanobody
   

   Tags

   Halo and His6

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   T7 promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3-Neo-EF1a-GFP

    Plasmid

    #69134

    Purpose

    Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

    Depositor

    Martin Bonamino

    Article

    Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

    Type

    Empty backbone

    Use

    Sleeping beauty transposon

    Expression

    Mammalian

    Promoter

    U3MSCV

    Available Since

    Jan. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-SpCas9 blast

    Plasmid

    #104997

    Purpose

    This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMGF196

    Plasmid

    #97005

    Purpose

    Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    LEMD2 (LEMD2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV delta 5

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. wt dynamin 2 pEGFP

    Plasmid

    #34686

    Depositor

    Sandra Schmid

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406G, V664A, H692R and Q758R

    Promoter

    CMV

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. YFP-GID1

    Plasmid

    #37305

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    See comments
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

15. mCh-KIF5B*-strep

    Plasmid

    #120164

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMTL5

    Plasmid

    #127967

    Purpose

    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKM468-EGFP

    Plasmid

    #108434

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with EGFP.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-eGFP-4xGly-TEV-Flag-6xHis
    

    Use

    Orbit integrating plasmid

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tol2-zTRAP

    Plasmid

    #42236

    Depositor

    Stephen L Johnson

    Article

    Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

    Insert

    EGFP-Rpl10a (rpl10a Zebrafish)
    

    Tags

    EGFP

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-GFAP-hM3D(Gq)-mCherry

    Plasmid

    #50478

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter

    Depositor

    Bryan Roth

    Has Service

    AAV5

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSG5 PPAR alpha

    Plasmid

    #22751

    Depositor

    Bruce Spiegelman

    Insert

    PPARalpha (Ppara Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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