20,062 results

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pLNHA-C1-HsXRN1

Plasmid

#66596

Purpose

expresses lambdaN-HA-tagged XRN1 in mammalian cells

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

XRN1 (XRN1 Human)

Tags

lambdaN-HA

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Article

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Expression

Bacterial and Yeast

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMUH_unc84_2XGFP

Plasmid

#46023

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

2XGFP

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-DAPK1

Plasmid

#116727

Purpose

Lentiviral expression of DAPK1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DAPK1 (DAPK1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG dCas9-KRAB-2A-EGFP

Plasmid

#92396

Purpose

CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9_KRAB_2A_EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-HA-NEK7

Plasmid

#75142

Purpose

Expresses N-terminal HA-tagged NEK7 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:EGFP

Plasmid

#118850

Purpose

Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

mitfa: EGFP (mitfa Synthetic, Zebrafish)

Use

Zebrafish expression, tol6

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)

Use

Human homologous recombination donor

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA4

Plasmid

#26680

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 4 (KPNA4 Human)

Tags

T7

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a-Cas9-His

Plasmid

#98158

Purpose

Purification of Cas9 protein

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

Insert

NLS-Cas9-NLS

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NFIA+SOX9

Plasmid

#129455

Purpose

Inducible overexpression of NFIA and Sox9 in human cells.

Depositor

Su-Chun Zhang

Article

Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-

Insert

Sox 9 (SOX9 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadDE

Plasmid

#193837

Purpose

Express TadDE (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-DualEditor-Cas9D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV RasV12 Neo (w108-1)

Plasmid

#22259

Depositor

Eric Campeau

Insert

HRAS G12V (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V mutant

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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psPAX2-D64V-NC-COM

Plasmid

#131226

Purpose

Expressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)

Depositor

Baisong Lu

Article

Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

Insert

COM

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Snap-Omp25

Plasmid

#69599

Purpose

Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

Depositor

David Sabatini

Article

Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

Insert

Snap-Omp25

Expression

Mammalian

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJA2

Plasmid

#19492

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA2 (DNAJA2 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK3

Plasmid

#78815

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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GPRC5C-Tango

Plasmid

#66384

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPRC5C (GPRC5C Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
PET30B-PTEN

Plasmid

#20741

Depositor

Alonzo Ross

Article

Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

Insert

PTEN (PTEN Human)

Tags

6xHis

Expression

Bacterial

Available Since

April 2, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-i53

Plasmid

#92170

Purpose

recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

i53

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

UbvG08 with I44A mutation and no terminal Glycines

Promoter

CMV

Available Since

July 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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