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pDSAY

Plasmid

#62291

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-HA-CPDSalI

Plasmid

#38253

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pLentiN 11E6

Plasmid

#37448

Purpose

Lentiviral expression of HPV 11E6 in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 11E6

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
cFGF8

Plasmid

#22089

Depositor

Gail Martin

Article

Crossley et al Cell. 1996 Jan 12. 84(1):127-36.

Insert

FGF8 (FGF8 Chicken)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
HS-H3.3A-GFP

Plasmid

#8696

Depositor

Kami Ahmad

Article

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H3.3A (His3.3A Fly)

Tags

GFP

Expression

Insect

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

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gS gag LV

Plasmid

#12327

Depositor

Inder Verma

Article

Kootstra et al Proc Natl Acad Sci U S A. 2003 Feb 4. 10

Insert

gag/pol

Use

Lentiviral

Expression

Mammalian

Mutation

The CypA binding region of the WT gag was replace…

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-18

Plasmid

#79933

Purpose

PH-4GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-18

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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p3XFlag-YedK

Plasmid

#84567

Purpose

Expresses YedK in mammalian cells

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK

Tags

3xFlag and C-Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-AD-uvr8at

Plasmid

#44971

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

AD-UVR8 (UVR8 Mustard Weed)

Tags

NF-κB activation domain

Expression

Mammalian

Mutation

deleted Methionine 1

Promoter

pCMV

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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Hec1 mCherry Plk1 K82R

Plasmid

#45206

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted mCherry Plk1 K82R

Expression

Mammalian

Mutation

Plk1 K82R mutation

Promoter

T7

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pAcUW51-gE2

Plasmid

#11621

Depositor

Pamela Bjorkman

Article

Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

Insert

HSV-1 glycoprotein E

Tags

His

Expression

Insect

Mutation

deleted amino acids 391-550

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pMBPcyto-TEV-BseRI

Plasmid

#157739

Purpose

empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Type

Empty backbone

Tags

His10 + MBP (cytoplasmic expression; no signal se…

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12A

Plasmid

#87061

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12A (Trim12a Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12C

Plasmid

#87080

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12C (Trim12c Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pABCb10aa1-70-GFP

Plasmid

#19038

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

amino acids 1 - 70 of the targeting sequence of ABCb1

Tags

GFP

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
EP300

Plasmid

#74658

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

EP300 (EP300 Human)

Tags

His-6-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus Insulin 2

Plasmid

#25786

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

Insert

Insulin 2

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pRD323

Plasmid

#198044

Purpose

Expression of HMfA K31A E35A in E. coli

Depositor

Remus Dame

Article

Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

Insert

HMfA K31A E35A

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pNCMV 11E6

Plasmid

#37455

Purpose

Expression of HPV 11E6 in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 11E6

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pTK715

Plasmid

#114698

Purpose

Express NuMA(1-2115)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115)-RFP-Nano

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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