20,066 results

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pCMV-APOBEC1-YTHmut

Plasmid

#131637

Purpose

Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

Depositor

Kate Meyer

Article

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1-YTHmut-HA (YTHDF2 Human, Rat)

Tags

HA

Expression

Mammalian

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Promoter

CMV

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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SP1-SB100X

Plasmid

#154887

Purpose

Sleeping Beauty transposase fused to SP1

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

SP1-Sleeping Beauty

Expression

Mammalian

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_PLD1

Plasmid

#45268

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PLD1 (PLD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB wt

Plasmid

#19301

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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Cilantro 2

Plasmid

#74450

Purpose

Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

Plasmid

#175574

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHBS1147 GFP-TDP43

Plasmid

#118797

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-GFP

Plasmid

#78548

Purpose

Cas9 protein with GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB414

Plasmid

#74808

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 WT

Plasmid

#69824

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

Plasmid

#74309

Purpose

Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-Bcl2

Plasmid

#46971

Purpose

lentiviral expression of Bcl2

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

Bcl2 (BCL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

Plasmid

#171381

Purpose

Expression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized Cas13Y.1

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV, U6

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-TS2126 RNA Ligase

Plasmid

#119941

Purpose

Express TS2126 RNA ligase in E. coli

Depositor

Takashi Ito

Article

Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

Insert

TS2126 RNA ligase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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amilCP chromoprotein

Plasmid

#117847

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA Ef1alpha Puro-T2A-GFP

Plasmid

#111596

Purpose

sgRNA Ef1alpha Puro-T2A-GFP

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP5G

Plasmid

#31788

Purpose

a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

Depositor

Douglas Kim, Loren Looger, GENIE Project

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

GCaMP5G

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Puro

Plasmid

#125594

Purpose

Lentiviral expression of sgRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-ERBB2

Plasmid

#23888

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB2 (ERBB2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only