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We narrowed to 16,074 results for: grna

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Showing: 6701 - 6720 of 16074 results

  1. pYN2_1

    Plasmid
    #184757
    Purpose
    Plasmid for genome editing by CRISPR/Cas9
    Depositor
    Yvonne Nygård
    Publication
    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10
    Inserts
    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    Use
    Synthetic Biology
    Tags
    2xNLS
    Expression
    Bacterial and Yeast
    Promoter
    PGK1 and tRNA-Phe-HDV Ribozyme
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    184757-plasmid-map-sequence-id-421882

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  2. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid
    #129531
    Purpose
    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR
    Depositor
    Erik Sontheimer
    Publication
    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d
    Inserts
    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    Use
    AAV
    Tags
    FLAG/NLS
    Promoter
    U6 and cytomegalovirus-enhancer chicken β-actin p…
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129531-plasmid-map-sequence-id-255632

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  3. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid
    #124541
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Publication
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    Expression
    Mammalian
    Mutation
    WT, WT
    Promoter
    Minimal ADE
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124541-plasmid-map-sequence-id-242326

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  4. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid
    #124539
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Publication
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55A, d11-41
    Promoter
    Minimal ADE
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124539-plasmid-map-sequence-id-242319

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  5. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid
    #124537
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Publication
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55G, d11-41 C55G
    Promoter
    Minimal ADE
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124537-plasmid-map-sequence-id-242309

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  7. pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

    Plasmid
    #155307
    Purpose
    Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP
    Depositor
    Stanley Qi
    Publication
    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do
    Insert
    Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, EF1a
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155307-plasmid-map-sequence-id-442982

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  14. TRMPV-Hygro

    Plasmid
    #27996
    Purpose
    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.
    Depositor
    Scott Lowe
    Publication
    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.
    Insert
    TRMPV-Hygro (shLuci-1309)
    Use
    RNAi and Retroviral; Fluorescent reporters
    Expression
    Mammalian
    Available since
    March 21, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    27996-plasmid-map-sequence-id-13247

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  18. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-GFP

    Plasmid
    #255027
    Purpose
    Lentiviral plasmid designed to express a SaCas9 compatible gRNA expression cassette and dSaCas9-KRAB-T2A-GFP protein; Similar to Plasmid #162334, but with GFP instead of Puro
    Depositor
    Jonathan Ploski
    Insert
    dSaCas9
    Use
    CRISPR and Lentiviral
    Available since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255027-plasmid-map-sequence-id-509438

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  19. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68356-plasmid-map-sequence-id-119628

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  20. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

    Plasmid
    #126698
    Purpose
    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence
    Depositor
    Darrell Kotton
    Publication
    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting human SCGB3A2 locus
    Expression
    Mammalian
    Promoter
    Human U6
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126698-plasmid-map-sequence-id-245766

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Showing: 6701 - 6720 of 16074 results