We narrowed to 7,534 results for: ski

1. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

   Plasmid

   #197503

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABEpuro-ERBB2 A775_G776insV, G776C

   Plasmid

   #40979

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   A775_G776insV, G776C

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

   Plasmid

   #185917

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpRY-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDMJ2-SARS-CoV-2-Spike (EG.5)

   Plasmid

   #241114

   Purpose

   Expresses SARS-CoV-2 spike (EG.5) protein for creating pseudotyped virus

   Depositor

   Jeremy Luban

   Article

   Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

   Insert

   Spike (EG.5) (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   T19I, L24del, P25del, P26del, A27S, V83A, G142D, …

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-NES-ZapCV2

   Plasmid

   #222950

   Purpose

   Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

   Depositor

   Amy Palmer

   Article

   Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

   Insert

   NES-ZapCV2 (NES Synthetic)
   

   Tags

   ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

   Expression

   Mammalian

   Mutation

   ECFP gene is truncated 36 bp at 3' end. Venu…

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.3-NL63-D14

   Plasmid

   #172666

   Purpose

   encodes NL63 spike protein, for pseudovirus production

   Depositor

   David Nemazee

   Article

   Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

   Insert

   HCNV63gp2 (HCNV63gp2 human coronavirus NL63)
   

   Use

   Lentiviral and Retroviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   c-terminal deletion of 14aa

   Promoter

   CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCmini-iCAP-AAV.CAP-Mac

   Plasmid

   #200658

   Purpose

   Synthetic construct isolate AAV.CAP-Mac VP1 gene

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   Synthetic construct isolate AAV.CAP-Mac VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   7 amino acid insertion after Q588 of the AAV9 CAP…

   Promoter

   p41

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.3_SARS2_omicron BA.2

   Plasmid

   #183700

   Purpose

   expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

   Insert

   Spike (BA.2) (S SARS-CoV-2)
   

   Use

   Lentiviral and Retroviral

   Expression

   Mammalian

   Mutation

   c-terminal 18 aa deletion

   Promoter

   CMV

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

   Plasmid

   #208291

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpRY(D10A/…

   Promoter

   CMV and T7

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCXLE-SOX2-p2a-KLF4

    Plasmid

    #193292

    Purpose

    episomal hSK (expressing human SOX2 + KLF4)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    SOX2-p2a-KLF4 (SOX2, KLF4 Human)
    

    Expression

    Mammalian

    Mutation

    F2A of original pCXLE-SK vector was replaced with…

    Promoter

    CAG

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_DNAJC15_WT_V5

    Plasmid

    #82964

    Purpose

    Gateway Donor vector containing DNAJC15, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    DNAJC15 (DNAJC15 Human)
    

    Use

    Gateway entry vector

    Mutation

    R35G

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-Myc-TRIP12-CD

    Plasmid

    #241814

    Purpose

    Expresses Myc-tagged catalytic dead TRIP12 in mammalian cells.

    Depositor

    Ethan Lee

    Article

    Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

    Insert

    TRIP12 (TRIP12 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    catalytic dead (C2007A)

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.3_SARS2_omicron_BA.1

    Plasmid

    #180375

    Purpose

    Pseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike protein

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    Spike (omicron BA.1 variant) (S SARS-CoV-2)
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    c terminal D18

    Promoter

    CMV

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_EGFR_p.769_770insASV

    Plasmid

    #82880

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Mutation

    ASV insertion between AA 769 and 770

    Promoter

    None

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

    Plasmid

    #197501

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

    Plasmid

    #197507

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO-Tet-On-YAP1-shRNA2

    Plasmid

    #176847

    Purpose

    lentiviral vector for doxycycline-inducible human YAP1 knockdown

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    YAP1-Sh2 (YAP1 Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-45b-mCherry-NPM1

    Plasmid

    #194546

    Purpose

    Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    NPM1 (Npm1 Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

    Plasmid

    #197500

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge