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pFB1_hPMS2

Plasmid

#129425

Purpose

Expression human PMS2 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human PMS2 (PMS2 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAD-IRP

Plasmid

#220751

Purpose

Plasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Insert

Iron Regulatory Protein (ACO1 )

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pACT-Lex

Plasmid

#183149

Purpose

Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 AD

Use

Yeat two-hybrid

Tags

HA tag

Expression

Yeast

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pFRTpsiCHECK_SEAP

Plasmid

#154461

Purpose

Expression of SEAP and firefly luciferase

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

SEAP

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

Plasmid

#211843

Purpose

Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA and mCherry

Expression

Plant

Mutation

C492A

Promoter

UBIQUITIN10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1_hMLH1

Plasmid

#129426

Purpose

Expression human MLH1 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MLH1 (MLH1 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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NSarQ27G-VMAn-FKBP

Plasmid

#70230

Purpose

Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

alpha Sarcin (N-terminal portion)

Tags

FK506- and rapamycin-binding protein (FKBP), Link…

Expression

Mammalian

Mutation

Changed Gln 27 to Gly

Promoter

CMV

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

Plasmid

#171438

Purpose

Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-c-Jun

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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PX459-APP-KO

Plasmid

#176487

Purpose

To knockout APP gene

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

gRNA sequence

Use

CRISPR

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-6h-GST-TEVp

Plasmid

#172887

Purpose

Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Insert

TEV protease

Tags

GST solubility tag and HHHHHH

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-HA/Net1A

Plasmid

#28262

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1A (Net1 Mouse)

Tags

2x HA

Expression

Mammalian

Available Since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
GI-Gal4 DBD

Plasmid

#42500

Purpose

GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor

Depositor

Ricardo Dolmetsch

Article

Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

Insert

GIGANTEA fused with Gal4 DBD

Tags

Gal4 DNA binding domain (DBD)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pACU2_CD4-mIFP T2A HO1

Plasmid

#72441

Purpose

Expressing UAS-CD4-mIFP T2A HO1 in drosophila

Depositor

Xiaokun Shu

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CD4-mIFP T2A HO1

Expression

Insect

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Venus-CAAX

Plasmid

#87612

Purpose

Plasma membrane-targeted Venus (YFP).

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Venus-CAAX

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBEL1092

Plasmid

#195635

Purpose

Expression/purification of 6xHis-TEV-sfGFP

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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PKG I alpha WT

Plasmid

#16390

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

Plasmid

#171436

Purpose

Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-Lifeact

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSb_init containing the MBP sybody Sb_MBP#1

Plasmid

#132699

Purpose

expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

sybody_MBP#1

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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mKate1.31-pBAD

Plasmid

#54827

Purpose

Localization: Protein Expression Vector , Excitation: 588, Emission: 635. Also known as mKate2.

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Type

Empty backbone

Tags

6xHis and mKate1.31 (aka mKate2)

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pHRW-CRY2-VHH(GFP)

Plasmid

#126022

Purpose

express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHR-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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