We narrowed to 11,589 results for: EMB
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Plasmid#173165PurposeRetroviral vector to express sgRNA resistant human ACSL3DepositorInsertAcyl-CoA Synthetase Long Chain Family Member 3 (ACSL3 Human)
UseRetroviralMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable SinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA
Plasmid#170999PurposeHiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[R537A]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[R537A]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA4
Plasmid#171002PurposeHiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP108 Nanog-Venus-2a-mCherry
Plasmid#159743PurposeNanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.DepositorAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_dHMGA_pET28
Plasmid#167329PurposeExpresses TFAM dHMGA mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
Tags6XHisExpressionBacterialMutationTruncation mutant containing linker, HMGB, and C-…PromoterT7Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_tacI
Plasmid#155170PurposeBaSP and CuCBP co-expression: tacI promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_araBAD
Plasmid#155176Purposesee pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBICI_2_strong
Plasmid#155168Purposesee pBICI_strong: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
TB:2xP53_RE:MiniP
Plasmid#133881Purpose2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
px459-RhebL1 sgRNA
Plasmid#133769PurposeExpresses Cas9 and human RhebL1 sgRNADepositorInsertRhebL1 sgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P440L
Plasmid#132596PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutationDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
phNFL-N98S
Plasmid#132592PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutationDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL942
Plasmid#119721Purposeprecursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 CysteineDepositorInsertprecusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C
TagsnoneExpressionBacterialMutationAlanine 248 to Threonine, Alanine 188 to Cysteine…PromoterT7Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBV163
Plasmid#108364PurposeUsed to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.DepositorInsertKnock-in Cg TDH3 promoter in Cg YAP1 gene
UseReplace c.glabrata yap1 promoter with c. glabrata…PromoterTDH3Available SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-B8I085
Plasmid#103089PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB8I085(Cas9 coding gene from Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/181/666A
Plasmid#105310PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-GEI-17(133-509)
Plasmid#87056PurposeExpresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORFDepositorInsertGEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)
Tags6xHis-TEV and GAGGGS linkerExpressionBacterialPromoterT7Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD3C
Plasmid#86971PurposeExpresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 fused to phosphite dehydrogenase and P450cam
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R in PCNA3, silent mutat…PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX4-AS-short (pcDNA3.1+)
Plasmid#75210PurposeMammalian expression of SLC24A4DepositorInsertSLC24A4 (SLC24A4 Human)
ExpressionMammalianMutationalternatively spliced exon absentPromoterCMVAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Jam4.1-Fc-His
Plasmid#72081PurposeExpresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only