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pYPQ142-ZmUbi-tRNA

Plasmid

#158578

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-sgRNA(siteA)

Plasmid

#154831

Purpose

sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cells

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

sgRNA(siteA)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pC1.509

Plasmid

#203955

Purpose

Level 1 self-replicating vector containing a DAPG inducible FnCas12a

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

PphlF-Cpf1

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFPL-Rh

Plasmid

#61649

Purpose

Gateway vector for generating red fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

monomeric red fluorescent protein

Expression

Plant

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Brec1

Plasmid

#123135

Purpose

Mammalian expression vector for Brec1.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Brec1 recombinase

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCBI-CRY2PHR-VPR

Plasmid

#192606

Purpose

Plasmid for constitutive expression of CRY2PHR-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

CRY2PHR-VPR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pKIR1.0

Plasmid

#86409

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Promoter

AtRPS5A

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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IDP432

Plasmid

#87000

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

SNAP-Nlg1AB-1xλN(i)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFsynW TeTx-LC

Plasmid

#197284

Purpose

Encodes for viral expression of the light chain of tetanus toxin.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

Tetanus toxin light chain

Use

Lentiviral

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS485

Plasmid

#188890

Purpose

Worm Cas9 expression vector, Peft-3 promoter

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Peft-3::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-CitronRH

Plasmid

#158545

Purpose

Mammalian expression of nonbinding variant of direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

CitronRH

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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sfTq2ox-N1

Plasmid

#117930

Purpose

Superfolder mTurquoise2 ox (pNM113)

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2ox

Expression

Mammalian

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.4

Plasmid

#235055

Purpose

Expresses Tad-CBE2.4 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.4-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMLS714

Plasmid

#188886

Purpose

MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_Uricase

Plasmid

#182400

Purpose

Bacterial expression of uricase

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

uricase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBWB294

Plasmid

#140636

Purpose

pBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Expression

Bacterial

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-H2B_Halo_T2A_EGFP

Plasmid

#135444

Purpose

Mamalian cell expression of nuclear HaloTag7 and cytosolic EGFP

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

H2B-HaloTag7 and EGFP

Tags

H2B

Expression

Mammalian

Promoter

CMV/TetO

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP703

Plasmid

#79985

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Expression

Bacterial

Promoter

araB

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSW039

Plasmid

#140035

Purpose

KO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Yellow fluorescent protein

Expression

Bacterial

Promoter

cLac143

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.6

Plasmid

#235058

Purpose

Expresses Tad-CBE3.6 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.6-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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