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pcDNA3-ApaLID211A-HA

Plasmid

#211459

Purpose

Express mitochondrial localized ApaLI with D211A mutation

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-D211A

Tags

HA

Expression

Mammalian

Mutation

D211A

Promoter

CMV

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD_D4910

Plasmid

#127220

Purpose

Module D with ZmUbi1::BBM

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

BBM

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCL20C-ddIPpoI

Plasmid

#49053

Purpose

Expresses the restriction enzyme IPpoI under the control of Shield1

Depositor

Michael Kastan

Article

Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

Insert

DD-HA-ER-IPpoI

Use

Lentiviral

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMOD_C'0000a

Plasmid

#127226

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Expression

Plant

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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p3993 pET ubcH7

Plasmid

#8654

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH7 (UBE2L3 Human)

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pC.HA Vav1

Plasmid

#14553

Depositor

Joan Brugge

Article

Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

Insert

Vav1 (VAV1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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Venus-PTP1b

Plasmid

#234740

Purpose

Mammalian expression of endoplasmic reticulum marker Venus-PTPb

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-PTP1b

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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HDR-HaloPOLQ

Plasmid

#211637

Purpose

halo donor

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

Puro-LoxHaloTag

Tags

flag

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMKV060

Plasmid

#133315

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+

Expression

Plant

Promoter

AtUbi10

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_C'5014

Plasmid

#127219

Purpose

Module C' with Pnos::WUS2

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMM112

Plasmid

#127229

Purpose

35S::IPT::pinII terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

IPT

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-STRA8

Plasmid

#222585

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of STRA8, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

STRA8 (STRA8 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pFSy(1.1)GW

Plasmid

#27232

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pZZMP09

Plasmid

#231754

Purpose

Mammalian expression of SPO11

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11 (Spo11 Mouse)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_FLAG_MTA3

Plasmid

#31696

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Metastasis associated protein 3 (MTA3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBabePuro-hSLUG.ER.NoTag

Plasmid

#19293

Depositor

Eric Fearon

Article

Hajra et al Cancer Res. 2002 Mar 15. 62(6):1613-8.

Insert

SLUG (SNAI2 Human)

Use

Retroviral

Tags

mouse ERalpha ligand-binding domain

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pEPQD0CM0296

Plasmid

#162305

Purpose

Level 0 CDS part for MoClo assembly, AATG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

CDS, superfolder GFP, no stop codon

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMM107

Plasmid

#127227

Purpose

35S::IPT::pinII terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

IPT

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GW

Plasmid

#27230

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SYMPK

Plasmid

#222556

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SYMPK

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SYMPK (SYMPK Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pFCK(0.4)GW

Plasmid

#27229

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MSX1

Plasmid

#222557

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MSX1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MSX1 (MSX1 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTYF-Tretight-DsRed2

Plasmid

#19983

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

Tretight-DsRed2

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PRDM9

Plasmid

#222565

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PRDM9

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

PRDM9 (PRDM9 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRGM1

Plasmid

#48789

Purpose

HA-I-SceI expression plasmid, episomal

Depositor

Michael Glickman

Article

Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

Insert

I-SceI

Tags

HA tag

Expression

Bacterial

Promoter

MOP

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pPI896

Plasmid

#207453

Purpose

REMI integrated Expression for mNeonGreen-Nap1

Depositor

Rob Kay

Article

Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

Insert

Nap1

Use

Unspecified

Tags

mNeonGreen

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0245

Plasmid

#162284

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0729

Plasmid

#162316

Purpose

TEV protease plasmid for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-NET-TEVprotease

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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p3992 pET ubcH5

Plasmid

#8652

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH5 (UBE2D1 Human)

Expression

Bacterial

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pET28a-nGFPMNase

Plasmid

#187826

Purpose

express nGFPMNase protein in E coli

Depositor

Yikang Rong

Article

Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

Insert

nGFPMNASE

Tags

his tag

Expression

Bacterial

Promoter

T7 Promotor

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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