We narrowed to 5,783 results for: ATC

1. pLKO.1 puro shTEAD1/3/4-2

   Plasmid

   #193673

   Purpose

   Constitutive lentiviral expression of TEAD shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   TEAD
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCRISPRv2-Beclin1

   Plasmid

   #99574

   Purpose

   Expressed Cas9 with sgRNA targeting Beclin1

   Depositor

   Edward Campbell

   Article

   Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

   Insert

   gRNA targeting Beclin1 (BECN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AF1521-c003

   Plasmid

   #173111

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
   

   Use

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   KH-M1-L192

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT9675-CBE

   Plasmid

   #163007

   Purpose

   plasmid expressing CBE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   CBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDIRECT_23A

   Plasmid

   #91138

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAT9748-dCBE

   Plasmid

   #163008

   Purpose

   plasmid expressing CBE with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dCBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAT9624-BEAR-cloning

   Plasmid

   #162986

   Purpose

   BEAR target cloning plasmid with split EGFP

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDIRECT_21A

   Plasmid

   #91128

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28-sfGFP-DogTag Loop A

   Plasmid

   #171779

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-DogTag Loop A
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag and linkers inserted between residues Val2…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-h-mmPtch1-FL

    Plasmid

    #120889

    Purpose

    Expresses wildtype Ptch1 for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAT9658-sgRNA-mCherry

    Plasmid

    #162987

    Purpose

    sgRNA cloning plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBSc + MShal

    Plasmid

    #16166

    Depositor

    Larry Salkoff

    Article

    Pak et al Proc Natl Acad Sci U S A. 1991 May 15. 8

    Insert

    Mus musculus potassium voltage-gated channel (Kcnd1 Mouse)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

13. Dlx5

    Plasmid

    #15538

    Depositor

    John Rubenstein

    Article

    Zerucha et al J Neurosci. 2000 Jan 15. 20(2):709-21.

    Insert

    Dlx5 (Dlx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pET-mBoNT/F

    Plasmid

    #168015

    Purpose

    Expresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    MBP-BoNT/F-LC(1-428)-6xHis
    

    Use

    Tags

    Hisx6 and maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET24a HAB1 (172-511)

    Plasmid

    #177844

    Purpose

    Bacterial Expression of HAB1

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    Protein phosphatase 2C 16
    

    Use

    Tags

    6xHis-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAT9650-BEAR-GFP-preedited

    Plasmid

    #162992

    Purpose

    BEAR control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-CRY2(D387A)-mCherry

    Plasmid

    #58369

    Purpose

    Light insensitive CRY2 mutant

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (D387A) (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D387A

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiCRISPRv2-KLF7-1

    Plasmid

    #169797

    Purpose

    Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for KLF7 neighbouring region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. shCDK4/6(2)

    Plasmid

    #73555

    Purpose

    vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

20. SpyTag003-OspC

    Plasmid

    #159998

    Purpose

    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag003-OspC (20-209) C129S
    

    Use

    Tags

    His6-tag and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    C129S mutation improves expression level.

    Promoter

    T7

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZmUBQ:AVRa1-V1

    Plasmid

    #132357

    Purpose

    Expression of the Blumeria graminis f. sp. hordei effector AVRa1-V1 ∆SP from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Insert

    AVRa1-V1 ∆SP with stop codon
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pZmUBQ

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pASKt15C+SrtA7

    Plasmid

    #64986

    Purpose

    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, E105K, E108A, D16…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAPM-D4 miR30-TRIM28 ts3

    Plasmid

    #115864

    Purpose

    TRIM28 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    TRIM28 shRNA (TRIM28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid

    #160139

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZmUBQ:AVRa1

    Plasmid

    #132356

    Purpose

    Expression of the Blumeria graminis f. sp. hordei effector AVRa1 ∆SP from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Insert

    AVRa1 ∆SP with stop codon
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pZmUBQ

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLKO-STAG2 shRNA 3782

    Plasmid

    #31979

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pC1-FGB (FlincG2)

    Plasmid

    #49204

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    FlincG2 (PRKG1 Bovine, GFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LEP-shMLL-AF9.1039

    Plasmid

    #105566

    Purpose

    retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    shRNA targeting MLL part of MLL-AF9 fusion
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMOD_A0402

    Plasmid

    #91009

    Purpose

    Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    AtUbi10

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28-sfGFP-DogTag Loop B

    Plasmid

    #171780

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Asp1…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-CVm-PTEN

    Plasmid

    #168032

    Purpose

    Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-PTEN(M265-N329)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA-h-mmPtch1-FL-TagBFP

    Plasmid

    #120921

    Purpose

    Expresses BFP tagged Ptch1

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJG363

    Plasmid

    #91183

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_D10A+gNt_R2+donor
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJG370

    Plasmid

    #91187

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_D10A+gRNActrl+donor
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMOD_A0601

    Plasmid

    #91016

    Purpose

    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_D10A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    35S

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSpCas9(BB)-2A-GFP-G3BP2(2)

    Plasmid

    #136061

    Purpose

    G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCRISP_IS

    Plasmid

    #120425

    Purpose

    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

    Depositor

    Tamás Fehér

    Article

    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

    Insert

    CRISPR spacers targeting IS1, IS5, IS3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MS2-adRNA (5'UAG)

    Plasmid

    #170126

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(5'UAG)-MS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GAL4-mNFAT1(2-144)

    Plasmid

    #11108

    Purpose

    Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

    Depositor

    Anjana Rao

    Article

    Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Tags

    GAL4-DBD

    Expression

    Mammalian

    Mutation

    Contains NFAT1 amino acids 2-144; L78P and P140Q …

    Promoter

    Available since

    April 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge