We narrowed to 16,118 results for: form

1. ALIX(1-702)-mNeonGreen

   Plasmid

   #191193

   Purpose

   Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

   Depositor

   Jin Zhang

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   ALIX (1-702) (PDCD6IP Human, Synthetic)
   

   Tags

   6xHIS and mNeonGreen

   Expression

   Mammalian

   Mutation

   Deletion of ALIX C-terminal PRD.

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. YF-Cdc42(CA)

   Plasmid

   #20152

   Depositor

   Tobias Meyer

   Article

   Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

   Insert

   YFP-FKBP-Cdc42(CA) (CDC42 Human)
   

   Expression

   Mammalian

   Mutation

   Cdc42H was inserted into the multiple cloning sit…

   Available Since

   March 26, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSOMA-T679A-NLS

   Plasmid

   #118938

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
   

   Expression

   Plant

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MmSphk2

   Plasmid

   #118598

   Purpose

   Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   GAL1, 10

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-X1-Neo-GFP-ATL2-deltaTM

   Plasmid

   #109021

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL2-deltaTM (ATL2 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   truncated form without C-terminal ER transmembran…

   Promoter

   EIF-1a promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSP64T acvr1ba*

   Plasmid

   #185409

   Purpose

   Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   acvr1ba (acvr1ba Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Expression

   Mammalian

   Mutation

   changed Threonine 206 to Aspartic acid

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSOMA-T679A-NES

   Plasmid

   #118940

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
   

   Expression

   Plant

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-At_PRORP1-His

   Plasmid

   #67869

   Purpose

   bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

   Insert

   PRORP1 (PRORP1 Mustard Weed)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 36-572 (see comments)

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL2-basic mt E2F-luciferase

   Plasmid

   #133708

   Purpose

   Expression of E2F1-mt-luc.

   Depositor

   James A. DeCaprio

   Article

   Zalvide et al Mol Cell Biol 1998 Mar;18(3):1408-15.

   Insert

   E2F1 (E2F1 Human)
   

   Use

   Trxn factor bind site

   Expression

   Mammalian

   Mutation

   the three binding site copies are mutated form

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA4/TO-myc NES-mCE NLS-Flag

    Plasmid

    #82468

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Myc-NES-mCE-Flag (Rngtt HIV, Mouse)
    

    Tags

    Flag and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HOXA4 (murine) pET

    Plasmid

    #8488

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXA4 (Hoxa4 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Thr-2 changed to Ala-2 to form better ribosomal b…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. Cerulean(1-158) in pcDNAI/Amp

    Plasmid

    #60897

    Purpose

    This vector attaches Cerulean(1-158) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    Cerulean(1-158)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+ Zf nhsl1b-mNeongreen

    Plasmid

    #233883

    Purpose

    mNeongreen tagged form of zebrafish nhsl1b. For in vitro transcription (SP6) or expression through CMV.

    Depositor

    Nicolas David

    Article

    Escot et al Commun Biol. 2025 Feb 28;8(1):328. doi:

    Insert

    nhsl1b (nhsl1b Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    mNeongreen

    Expression

    Mammalian

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-SFFV-myc-ATL3-WPRE-UbC-Emerald

    Plasmid

    #225946

    Purpose

    Lentiviral vector plasmid expressing human atlastin GTPase 3 (ATL3) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Evan Reid, Richard Eva

    Inserts

    ATL3 (ATL3 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

    Plasmid

    #225949

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Evan Reid, Richard Eva

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C100A

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCit(S1h)3(S3h)3-gs

    Plasmid

    #228079

    Purpose

    Mammalian expression of coiled coil segments (S1h)x3-(S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCit, S1h, S3h
    

    Expression

    Mammalian

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TagBFP(S2-S4)1-gs

    Plasmid

    #228074

    Purpose

    Mammalian expression of coiled coil segments (S2-S4)x1, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    BFP, S2, S4
    

    Expression

    Mammalian

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCit(S1h-S3h)3-gs

    Plasmid

    #228076

    Purpose

    Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCit, S1h, S3h
    

    Expression

    Mammalian

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCit(D1h-D3h)3-gs

    Plasmid

    #228088

    Purpose

    Mammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCit, D1h, D3h
    

    Expression

    Mammalian

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCit(S1-S3)1-SAH

    Plasmid

    #228073

    Purpose

    Mammalian expression of coiled coil segments (S1-S3)x1, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCitrine, S1, S3
    

    Expression

    Mammalian

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mCit(S1-S3)3-gs

    Plasmid

    #228070

    Purpose

    Mammalian expression of coiled coil segments (S1-S3)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCitrine, S1, S3
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TagBFP(S2-S4)3-gs

    Plasmid

    #228075

    Purpose

    Mammalian expression of coiled coil segments (S2-S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    BFP, S2, S4
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TagBFP(S2h)3(S4h)3-gs

    Plasmid

    #228077

    Purpose

    Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    BFP, S2h, S4h
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNB_EA_R1CiM_4EGFP_R3EGFPi2

    Plasmid

    #185968

    Purpose

    Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

    Depositor

    Sangram Bagh

    Article

    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

    Insert

    Molecular IMPLY gate
    

    Use

    Synthetic Biology

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. tcTRP24sub8_SH2sub6

    Plasmid

    #175799

    Purpose

    de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing 2 copies of human Nck2 SH2

    Depositor

    Barry Stoddard

    Article

    Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

    Insert

    tcTRP24sub8_SH2sub6
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-SOMA-T679A

    Plasmid

    #118936

    Purpose

    Used to express the T679A mutant form of the SOMA FRET sensor in E. coli.

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMT2 Cav2.2 DomI and DomII

    Plasmid

    #89891

    Purpose

    Mammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2

    Depositor

    Annette Dolphin

    Article

    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

    Insert

    Cacna1b (CACNA1B )
    

    Expression

    Mammalian

    Mutation

    Stop codon after amino acid 1154 (this is a trunc…

    Promoter

    adenovirus major late promoter

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available Since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. CROPseq-Guide-Puro

    Plasmid

    #86708

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Christoph Bock

    Article

    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 8522-M01-663

    Plasmid

    #225660

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCopGFP
    

    Use

    Lentiviral

    Promoter

    SORE6-mCMVp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 8522-M31-663

    Plasmid

    #225661

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCherry
    

    Use

    Lentiviral

    Promoter

    SORE6-mCMVp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pQE-80L-V4-ELP-EEP13

    Plasmid

    #237223

    Purpose

    Expresses micelle-forming elastin-like polypeptide (ELP)-endosomal escape peptide (EEP) fusion construct in bacteria

    Depositor

    Elliot Chaikof

    Article

    Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

    Insert

    V4-ELP-EEP13
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. NET5s pEGFP-N2 (1154)

    Plasmid

    #62034

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET5s (TMEM201 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    short form

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

34. NET5L pEGFP-N2 (1155)

    Plasmid

    #62035

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    NET5L (TMEM201 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    long form

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pAAV-Syn-ERT2-iCre-ERT2

    Plasmid

    #178059

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human synapsin 1 (Syn) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLKO-TRE-AFOS

    Plasmid

    #197079

    Purpose

    A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

    Depositor

    Elaine Fuchs

    Article

    Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

    Insert

    AFOS (Fos Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and T7

    Promoter

    TRE

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. polyQ74-GFP

    Plasmid

    #231893

    Purpose

    Forms cytosolic HTT partial exon 1 Q74 aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pL-CRISPR.SFFV.GFP

    Plasmid

    #57827

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEF1a_CD WT TET1

    Plasmid

    #124082

    Purpose

    Expression of wild-type catalytic domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 catalytic domain (TET1 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    EF1 alpha

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBABE-puro TPR-Met

    Plasmid

    #10902

    Depositor

    Bob Weinberg

    Article

    Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

    Insert

    TPR-Met (MET, TPR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Constitutively active form of the c-Met receptor

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid

    #55201

    Purpose

    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pPFO*

    Plasmid

    #120400

    Purpose

    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

    Depositor

    Arun Radhakrishnan

    Article

    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

    Insert

    Mutant form of Perfringolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Amino acids 1-27 are deleted, starts at amino aci…

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. CROPseq-Guide-BSD

    Plasmid

    #216123

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. pL-CRISPR.SFFV.tRFP

    Plasmid

    #57826

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-tRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAAV-UBC-ERT2-iCre-ERT2

    Plasmid

    #178060

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid

    #55200

    Purpose

    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBtPIPLC His

    Plasmid

    #173808

    Purpose

    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

    Insert

    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pQHA-USP11 CS puroR

    Plasmid

    #46750

    Purpose

    Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

    Depositor

    Gordon Peters, Goedele Maertens

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. TRE3G-CTCF-dRBR-HA-MND-zeo

    Plasmid

    #232802

    Purpose

    Inducible over expression of RNA-binding domain deficient form of CTCF.

    Depositor

    Chunliang Li

    Article

    Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

    Insert

    Ctcf-dRBR (Ctcf Mouse)
    

    Use

    Lentiviral

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

    Plasmid

    #225947

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Evan Reid, Richard Eva

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S3E S17E S19E

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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