We narrowed to 2,540 results for: organization Addgene

1. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

   Plasmid

   #32702

   Purpose

   Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Insert

   eGFP
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IP-EGFP-mouse Plaat3 (WT)

   Plasmid

   #167987

   Purpose

   Expresses EGFP-Plaat3 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   Hrasls3 (Plaat3 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-TRE-SHH

   Plasmid

   #134350

   Purpose

   Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

   Depositor

   Lorenz Studer

   Article

   Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

   Insert

   SHH (SHH Human)
   

   Expression

   Mammalian

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-HSPA8

   Plasmid

   #121161

   Purpose

   Mammalian expression of GFP tagged HSPA8

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   HSPA8 (HSPA8 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS1NB m Rosella I

   Plasmid

   #71247

   Purpose

   a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.

   Depositor

   Mark Prescott

   Article

   Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

   Insert

   Rosella
   

   Tags

   yeast mitochondrial targeting sequence of citrate…

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPLICS Po-Mt Long P2A

   Plasmid

   #164115

   Purpose

   Detect the long Peroxisomes-mitochondria contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Po-Mt Long P2A
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pfa6a mScarlet-FKBP::KANMX

   Plasmid

   #185905

   Purpose

   Plasmid for cloning mScarlet fused to FKBP into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   mScarlet-FKBP
   

   Tags

   mScarlet fused to FKBP

   Expression

   Yeast

   Promoter

   SP6

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE60 R130A wgMDH

   Plasmid

   #204266

   Purpose

   Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R130A

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE60 R124A wgMDH

   Plasmid

   #204257

   Purpose

   Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124A

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LifeAct-GFP-Fis1

    Plasmid

    #182576

    Purpose

    Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    LifeAct-GFP-Fis1
    

    Tags

    Fis1 and LifeAct

    Expression

    Mammalian

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE60 R196A wgMDH

    Plasmid

    #204271

    Purpose

    Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-Dcp1b

    Plasmid

    #153976

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1b

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA recapping enzybe 1b (DCP1B Human)
    

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ZJOM65

    Plasmid

    #133654

    Purpose

    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ZJOM135

    Plasmid

    #133659

    Purpose

    Integration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SEC63-2mTagBFP2 (SEC63 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_iMYOD1_P2A_GFP_Puro

    Plasmid

    #168804

    Purpose

    PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    MYOD1 (MYOD1 Human, Synthetic)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ZJOM82

    Plasmid

    #133648

    Purpose

    Integration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-NAB2-mCherry (NAB2 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZJOM64

    Plasmid

    #133655

    Purpose

    Integration of COX4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX4-DuDre (COX4 Budding Yeast)
    

    Tags

    DuDre

    Expression

    Yeast

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. SNAP-Dcp2

    Plasmid

    #153975

    Purpose

    SNAP-tag polypeptide fused to mRNA decapping enzyme 2

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA Decapping enzyme 2 (DCP2 Human)
    

    Tags

    SNAP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pfa6a PhoCl-TsCC(B)::KANMX

    Plasmid

    #185906

    Purpose

    Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    PhoCl-TsCC(B)
    

    Tags

    PhoCl fluorophore with C-terminal TsCC(B)

    Expression

    Yeast

    Promoter

    SP6

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

    Plasmid

    #196192

    Purpose

    human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
    

    Use

    Znf donor

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pfa6a mScarlet-TsCC(B)::KANMX

    Plasmid

    #176397

    Purpose

    Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-TsCC(B)
    

    Tags

    mScarlet fused to TsCC(B)

    Expression

    Bacterial and Yeast

    Promoter

    none

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SPLICS Po-Mt Short P2A

    Plasmid

    #164116

    Purpose

    Detect the short Peroxisomes-mitochondria contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Short P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQE60 Q58L wgMDH

    Plasmid

    #204246

    Purpose

    Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Q58L

    Promoter

    T5

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.tgoGAP1

    Plasmid

    #78737

    Purpose

    Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

    Depositor

    Teresa Alonso

    Article

    Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Insert

    tgoGAP1
    

    Tags

    N terminal 81 amino acids of human galactosyltran…

    Expression

    Mammalian

    Mutation

    D117A/D119A/D163A in aequorin moiety

    Promoter

    CMV

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pET22b_PstS_3

    Plasmid

    #78200

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,I76G,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C,I76G,A197C (please see depositor comment bel…

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET_SZ1-RGG-RGG

    Plasmid

    #124942

    Purpose

    Expression of SZ1-RGG-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167942

    Purpose

    Inducible NKX2-1 overexpression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    TTF1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Promoter

    tetON

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ZJOM143

    Plasmid

    #133667

    Purpose

    Integration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET_RGG-GFP-RGG

    Plasmid

    #124948

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    EGFP and His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PB_iMYOD1_P2A_BAF60C2_T2A_mCh_Puro

    Plasmid

    #168807

    Purpose

    PiggyBac transposon construct with doxycycline inducible MYOD1, BAF60C + mCherry expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    MYOD1 (MYOD1 Human, Synthetic)
    

    Use

    Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ZJOM68

    Plasmid

    #133657

    Purpose

    Integration of TGL3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-TGL3-mCherry (TGL3 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. RA-Sec61β

    Plasmid

    #153978

    Purpose

    Dimerization dependent fluorescent protein RA domain fused to ER protein Sec61β

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    Sec61 Transolocon Subunit Beta (SEC61B Human)
    

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ZJOM119

    Plasmid

    #133661

    Purpose

    Integration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-NAB2-mTagBFP2 (NAB2 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. BFP-Dcp1a

    Plasmid

    #153973

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Tags

    mTagBFP-c1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GFP-LiMETER-v2

    Plasmid

    #113934

    Purpose

    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    LOV2 and Stim1 polybasic domain (PB)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ZJOM149

    Plasmid

    #133646

    Purpose

    Integration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ELO3-mCherry (ELO3 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET22b_PstS_2

    Plasmid

    #78199

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C; A197C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA_RGG-GFP-RGG

    Plasmid

    #124930

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. linker-mCitrine-PIF6-NAT

    Plasmid

    #51576

    Purpose

    Tag protein of interest with PIF6 in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PIF6 (PIL2 Mustard Weed)
    

    Tags

    linker and mCitrine

    Expression

    Yeast

    Mutation

    1-100aa of PIF6

    Promoter

    none

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMXs-IP-PEX3-EGFP

    Plasmid

    #228142

    Purpose

    Expresses PEX3-GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    PEX3 (PEX3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. PhyB-mCherry-PEX3

    Plasmid

    #51572

    Purpose

    Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    PEX3 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET_RGG-RGG

    Plasmid

    #124941

    Purpose

    Expresion of RGG-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Calnexin-HT-mGold

    Plasmid

    #183987

    Purpose

    Expresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    Calnexin (CANX Human)
    

    Tags

    HaloTag, mGold

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. GFP-BICDN-CIBN

    Plasmid

    #102254

    Purpose

    Expresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    GFP-BICDN-CIB1 (CIB1 Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLaNGo11-7

    Plasmid

    #126055

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pET_MBP-RGG-RGG

    Plasmid

    #124946

    Purpose

    Expression of MBP-RGG-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. ZJOM30

    Plasmid

    #133673

    Purpose

    Integration of ERG6-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on ER and lipid droplets.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ERG6-GFP (ERG6 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. GB-Dcp1b

    Plasmid

    #153979

    Purpose

    Dimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1b

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1b (DCP1B Human)
    

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. ZJOM75

    Plasmid

    #133649

    Purpose

    Integration of ANP1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ANP1-mCherry (ANP1 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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