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Showing: 661 - 680 of 18305 results

  1. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

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  2. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Qiao Zhou
    Article
    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61041-plasmid-map-sequence-id-456476

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  3. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid
    #61362
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61362-plasmid-map-sequence-id-99796

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  4. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  5. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  6. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61361-plasmid-map-sequence-id-99794

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  7. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  8. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

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  9. pZH_tdPP7cp_mCherry

    Plasmid
    #118267
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-mCherry
    Depositor
    Zach Hensel
    Insert
    tdPP7cp-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118267-plasmid-map-sequence-id-229509

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  10. pZH_mPP7cp_mCerulean3

    Plasmid
    #118262
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-mCerulean3
    Depositor
    Zach Hensel
    Insert
    mPP7cp-mCerulean3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118262-plasmid-map-sequence-id-229514

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  11. pZH_tdPP7cp_mCerulean3

    Plasmid
    #118263
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-mCerulean3
    Depositor
    Zach Hensel
    Insert
    tdPP7cp-mCerulean3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118263-plasmid-map-sequence-id-229501

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  12. pZH_mPP7cp_mCherry

    Plasmid
    #118266
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-mCherry
    Depositor
    Zach Hensel
    Insert
    mPP7cp-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118266-plasmid-map-sequence-id-229515

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  13. pZH_tdPP7cp_ECFP

    Plasmid
    #118265
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-ECFP
    Depositor
    Zach Hensel
    Insert
    tdPP7cp-ECFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118265-plasmid-map-sequence-id-229511

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  14. pZH_mPP7cp_ECFP

    Plasmid
    #118264
    Purpose
    Low-noise, tetracycline inducible expression of mPP7cp-ECFP
    Depositor
    Zach Hensel
    Insert
    mPP7cp-ECFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO-1
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118264-plasmid-map-sequence-id-229507

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  15. pJBL7058

    Plasmid
    #167227
    Purpose
    overexpression of orthogonal T7 RNAP
    Depositor
    Julius Lucks
    Insert
    J23119-AKSIRV
    Use
    Synthetic Biology
    Tags
    6X His
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167227-plasmid-map-sequence-id-332706

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  16. pJBL7055

    Plasmid
    #167224
    Purpose
    orthogonal T7 RNAP sfGFP reporter
    Depositor
    Julius Lucks
    Insert
    pRV-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167224-plasmid-map-sequence-id-331030

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  17. pJBL7057

    Plasmid
    #167226
    Purpose
    orthogonal T7 RNAP sfGFP reporter
    Depositor
    Julius Lucks
    Insert
    pIRH-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167226-plasmid-map-sequence-id-331033

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  18. pJBL7065

    Plasmid
    #167234
    Purpose
    cell-free cascaded nitrate sensor
    Depositor
    Julius Lucks
    Insert
    pNar-AKSIRV
    Use
    Synthetic Biology
    Tags
    6X His
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167234-plasmid-map-sequence-id-331128

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  19. pJBL7066

    Plasmid
    #167235
    Purpose
    cell-free cascaded mercury sensor
    Depositor
    Julius Lucks
    Insert
    pMer-AKSIRV
    Use
    Synthetic Biology
    Tags
    6X His
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167235-plasmid-map-sequence-id-331129

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  20. pJBL7069

    Plasmid
    #167238
    Purpose
    malachite green aptamer tagged sfGFP
    Depositor
    Julius Lucks
    Insert
    J23119-sfGFP-MGA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167238-plasmid-map-sequence-id-331131

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Showing: 661 - 680 of 18305 results