We narrowed to 18,872 results for: Tec

1. pFEndoNA2

   Plasmid

   #174667

   Purpose

   Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

   Depositor

   Jukka Finne

   Article

   Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

   Insert

   GFP-endoNA2 fusion protein
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. FpG5

   Plasmid

   #69443

   Purpose

   lentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAs

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Inserts

   minimal FGF4 promoter
   EGFP
   Ubiquitin promoter
   Hygromycin resistance
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-C1-MKLP1

   Plasmid

   #70154

   Purpose

   mammalian expression of MKLP1 with an mCherry fusion

   Depositor

   Masanori Mishima

   Article

   Joseph et al EMBO J. 2012 May 30;31(11):2604-14. doi:

   Insert

   MKLP1 (KIF23 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pExpreS2-1-C3-10H-SIII

   Plasmid

   #175444

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Tags

   3C-10His-TwinStrep and BiP Secretion Peptide

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-DsRed2

   Plasmid

   #112219

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   DsRed2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   DsRed2 contains a silent nucleotide substitution …

   Available Since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-BBS2

   Plasmid

   #218712

   Purpose

   Expresses N-terminally EGFP-tagged BBS2 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

   Insert

   BBS2 (BBS2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-Flag-hOct4

   Plasmid

   #20072

   Depositor

   Juan Belmonte

   Article

   Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

   Insert

   hOct4 (POU5F1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

   Plasmid

   #216696

   Purpose

   Synchronize trafficking of TNFalpha from the ER (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   Tumor Necrosis Factor alpha (TNFa)
   

   Tags

   FIREtag, Streptavidin Binding Protein (SBP), and …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pExpreS2-1-C3-PA-10H-SIII

   Plasmid

   #175443

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Tags

   3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHLuc (Antares-SEPLuc C1A4E+6)

    Plasmid

    #141089

    Purpose

    final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZIKV_Sensor_27B_LacZ

    Plasmid

    #75006

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_27B_LacZ
    

    Tags

    LacZ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCD86-EGFP

    Plasmid

    #133858

    Purpose

    Mammalian expression of CD86 C-terminally tagged with EGFP

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    CD86 (CD86 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG-VSVG

    Plasmid

    #80606

    Purpose

    Mammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the protein

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    VSV-G
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-BBS7

    Plasmid

    #218715

    Purpose

    Expresses N-terminally EGFP-tagged BBS7 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS7 (BBS7 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-GNB1

    Plasmid

    #133856

    Purpose

    Mammalian expression of GNB1 N-terminally tagged with EGFP

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    GNB1 (GNB1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-C1-BBS5

    Plasmid

    #218714

    Purpose

    Expresses N-terminally EGFP-tagged BBS5 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS5 (BBS5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-EGFP-HseIF4E

    Plasmid

    #79437

    Purpose

    Expresses EGFP-tagged HseIF4E in Mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    HseIF4E (EIF4E Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmApple-GNG2

    Plasmid

    #133857

    Purpose

    Mammalian expression of GNG2 N-terminally tagged with mApple

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    GNG2 (GNG2 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-Tight-Puro_Olig1

    Plasmid

    #64841

    Purpose

    A lentiviral vector for the expression of mouse Olig1 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Olig1 (Olig1 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBacDual with LFn + Needle

    Plasmid

    #84868

    Purpose

    Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Needle
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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