159,157 results

1. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

   Plasmid

   #209079

   Purpose

   Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   sfGFP
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_SFFv_4D5-WT-Highest-CAR_RHL004

   Plasmid

   #164826

   Purpose

   Lentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CAR

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   AntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61593

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   HA, NLS, and OLLAS tag

   Expression

   Mammalian

   Mutation

   K175R and K736R (deUb mutations)

   Promoter

   Available since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPGC1a_Distal Promoter-FLuc2P

   Plasmid

   #154260

   Purpose

   Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   PGC-1a Distal Promoter (PPARGC1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGC-1alpha Distal Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

   Plasmid

   #216531

   Purpose

   Targeting vector for CRISPRd generation

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   ABI-dCas9-mCherry
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-Myc3-Nrf2

   Plasmid

   #21555

   Purpose

   Mammalian expression of human Nrf2 with Myc tag

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

   Insert

   nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. ERroGFP_iE_pCDNA3

   Plasmid

   #47954

   Purpose

   Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged redox probe roGFPiE (roGFP C147, E147b, C204)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   ERroGFP
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   insertion E147B

   Promoter

   Available since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-C1-GOLGA4-GRIP

   Plasmid

   #220068

   Purpose

   overexpreeses the TGN protein GRIP with a mCherry fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

   Insert

   GOLGA4-GRIP (GOLGA4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2398

   Plasmid

   #142066

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZFPM2 (ZFPM2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-puro/Flag PD-L1 WT

    Plasmid

    #121446

    Purpose

    expression of huamn PD-L1 wildtype in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    wildtype

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER20-Flag-hSPDEF WT

    Plasmid

    #100873

    Purpose

    Lentiviral expression of human SPDEF

    Depositor

    Noah Shroyer

    Article

    Lo et al Gastroenterology. 2017 Jul;153(1):205-21

    Insert

    SPDEF (SPDEF Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE2

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Use

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid

    #50831

    Purpose

    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

    Depositor

    Peter Schultz

    Article

    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Inserts

    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    

    Use

    Baculoviral

    Tags

    His, Myc-6xHis, and WPRE

    Expression

    Insect

    Mutation

    Y39TAG and able to charge various unnatural amino…

    Promoter

    CAG, CMV, and U6 and H1

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

    Plasmid

    #45574

    Purpose

    Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    co I-Sce I
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDB_053

    Plasmid

    #216083

    Purpose

    for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
    

    Use

    Destination

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCB301-2μ-HDV

    Plasmid

    #196329

    Purpose

    A yeast-Escherichia coli-Agrobacterium shuttle vector

    Depositor

    Zhenghe Li

    Article

    Sun et al Viruses. 2017 Nov 7;9(11):332. doi: 10.3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTagRFP-T-Endosomes-14

    Plasmid

    #58014

    Purpose

    Localization: Endosomes, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Endosomes (RHOB Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. TFORF0753

    Plasmid

    #143438

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIVEP3 (HIVEP3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEYFP-N1-Pink1

    Plasmid

    #101874

    Purpose

    Transient expression of Pink1-YFP in mammalian cells

    Depositor

    Richard Youle

    Article

    Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

    Insert

    PTEN induced putative kinase 1 (PINK1 Human)
    

    Use

    Tags

    fluorescent tag EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. TfR-pHoran4

    Plasmid

    #61547

    Purpose

    Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Use

    Tags

    pHoran4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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