165,567 results

1. pUC118-mCtnnb1-FLIP-FlpE-Puro

   Plasmid

   #84541

   Purpose

   CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   mouse beta-catenin FLIP-FlpE - Puro targeting vector
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-CXCL12-sfGFP

   Plasmid

   #98961

   Purpose

   Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCL12 (CXCL12 Human)
   

   Tags

   Superfolder GFP

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLC242-GFP-Sestrin2

   Plasmid

   #100519

   Purpose

   Expresses GFP tagged Sestrin2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   SESN2 (SESN2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1+-Tornado-nLuc

   Plasmid

   #212611

   Purpose

   For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-nLuc
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG5-BSLF1

   Plasmid

   #69834

   Purpose

   Mammalian expression vector for Epstein-Barr virus primase BSLF1

   Depositor

   S. Diane Hayward

   Article

   Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

   Insert

   BSLF1
   

   Expression

   Mammalian

   Mutation

   S2F

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pDONR223-RPS6KA5

   Plasmid

   #23758

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   RPS6KA5 (RPS6KA5 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

7. TfR-sfGFP-myc tag-SpyCatcher003

   Plasmid

   #133451

   Purpose

   Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   TfR-sfGFP-myc tag-SpyCatcher003
   

   Tags

   GSSGS and myc tag

   Expression

   Mammalian

   Mutation

   Contains C20 and A23 mutations that improve plasm…

   Promoter

   CMV

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGM1190

   Plasmid

   #69994

   Purpose

   OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aac
   tsr
   rep
   sso
   TipA promoter
   dso
   

   Expression

   Bacterial

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PPIC

   Plasmid

   #25606

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PPIC (PPIC Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 30, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pSG5-BBLF2/3

    Plasmid

    #72633

    Purpose

    Mammalian expression vector for Epstein-Barr virus primase associated factor

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BBLF2/3
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. EGFP-RILP

    Plasmid

    #110498

    Purpose

    Fluorescent RILP

    Depositor

    Terence Dermody, Bernardo Mainou

    Article

    Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

    Insert

    RILP (RILP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMTU-DO-HIS-C4

    Plasmid

    #160340

    Purpose

    High-copy expression vector for K. marxianus, histidine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PTK2BA

    Plasmid

    #42401

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTK2BA (PTK2B Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. CDH5-AbR-GFP

    Plasmid

    #122970

    Purpose

    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

    Depositor

    Budd A. Tucker

    Inserts

    CDH5p
    CopGFP
    Zeocin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNO286-3

    Plasmid

    #107746

    Purpose

    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

    Depositor

    Jeffrey Tabor

    Article

    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Deleted AA 223 and 512

    Promoter

    J23106 and J23108

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-STONIN2

    Plasmid

    #177885

    Purpose

    Espresses Stonin2 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Martina et al J Cell Biol. 2001 May 28;153(5):1111-20.

    Insert

    stonin2 (STON2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. APP_Halo_C_allele

    Plasmid

    #178137

    Purpose

    Donor vector for endogenous tagging of human APP at the C-terminus with halotag

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Halotag (APP Human)
    

    Use

    Donor vector

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5FRT/TO_HA-mAID-nanobody

    Plasmid

    #117713

    Purpose

    Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mAID-nanobody
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMTU-DO-LEU-C4

    Plasmid

    #160341

    Purpose

    High-copy expression vector for K. marxianus, leucine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSG5-BALF2

    Plasmid

    #72629

    Purpose

    Mammalian expression vector for Epstein-Barr virus single-strand DNA binding protein

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BALF2
    

    Expression

    Mammalian

    Mutation

    S1028P and V1088A

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only