We narrowed to 15,686 results for: genome

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pTransgenesis p3 sv40 minimal promoter:: Katushka RFP

Plasmid

#131731

Purpose

Transposon based transgenesis

Depositor

Rene Maehr

Article

Stanney W et al Dev Biol. 2020 Mar 15;459(2):161-180. do

Insert

SV40MinPromoter

Use

Xenopus expression

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pegRNA_HEK4_20-7

Plasmid

#201974

Purpose

Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

pegRNA_HEK4_20-7

Expression

Mammalian

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-MPHOSPH8 Chromo

Plasmid

#59694

Purpose

Contains histone modification interacting domain (MPHOSPH8 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

MPHOSPH8 Chromo (MPHOSPH8 Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only

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AAEL003877-Cas9

Plasmid

#100581

Purpose

The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL003877-hspcas9-GFP
Opie-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL003877 and Opie2

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEPQD2KN0897

Plasmid

#177090

Purpose

MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

Plasmid

#172226

Purpose

Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

SOX17 (SOX17 Human)

Use

Piggybac transposon

Tags

3XFLAG, DD-domain, GS-linker, and TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMA7

Plasmid

#79967

Purpose

TM-MAGE strain

Depositor

Morten Sommer

Article

Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

Inserts

Lambda Red recombinase beta subunit
DNA adenine methylase

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_KDM6A_2

Plasmid

#86308

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA scaffold-spacer-human U6

Plasmid

#214697

Purpose

provide "sgRNA scaffold-spacer-human U6" fragment

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

sgRNA scaffold-spacer-human U6

Use

CRISPR

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pPGP_scFv

Plasmid

#183732

Purpose

Expresses anti-lysozyme scFv antibody

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Promoter

GAP promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFETCh_FOXP1

Plasmid

#86261

Purpose

Donor vector for 3' FLAG tag of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXP1 homology arms (FOXP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJEP301-pAAV-CMV-MCS3-pA

Plasmid

#113676

Purpose

CMV driven Multi Cloning Site-3

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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MSP2372

Plasmid

#73196

Purpose

Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

R661A, Q695A, and Q926A in SpCas9

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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px330 RBP1 gRNA

Plasmid

#165593

Purpose

Insertion of RBP1 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

RBP1 (Polr2a Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_791

Plasmid

#216079

Purpose

Minimal plasmid; contains only ori and ampR, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Destination

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AA163

Plasmid

#216061

Purpose

Fragmid fragment: (2A Selection) Stop only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-HA-dSpCas9

Plasmid

#92112

Purpose

Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9

Use

CRISPR

Tags

HA tag and NLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE WASF1-GFP KI

Plasmid

#131502

Purpose

Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Wasf1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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AA082

Plasmid

#215997

Purpose

Fragmid fragment: (Selection) fluorescent reporter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; mTagBFP2_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFETCh_KAT7

Plasmid

#86266

Purpose

Donor vector for 3' FLAG tag of human KAT7

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KAT7 homology arms (KAT7 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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