We narrowed to 76,952 results for: Rest

1. pCer-C3-Plk1-T210D

   Plasmid

   #68133

   Purpose

   Expression of Cerulean-tagged, constitutively active Plk1 in mammalian cells

   Depositor

   Catherine Lindon

   Insert

   Polo-like kinase 1 (PLK1 Human)
   

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   T210D constitutively active version

   Promoter

   CMV

   Available Since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCM351

   Plasmid

   #46013

   Depositor

   Mary Lidstrom

   Article

   Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

   Type

   Empty backbone

   Use

   Cre/Lox

   Promoter

   na

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-miRFP670

   Plasmid

   #91854

   Purpose

   Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

   Depositor

   Ralf Kuehn

   Inserts

   hSpCas9-2A-miRFP670
   BB-guide RNA
   

   Use

   CRISPR

   Tags

   2A-miRFP670 and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh and U6

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. plenti guide Thy1.1

   Plasmid

   #128063

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX551-CMV-SpCas9

   Plasmid

   #107024

   Purpose

   AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab

   Depositor

   Alex Hewitt

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS mFgfr1 (CT#92)

   Plasmid

   #14005

   Depositor

   Cliff Tabin

   Insert

   Fgfr1 (Fgfr1 Mouse)
   

   Expression

   Bacterial

   Available Since

   Feb. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

7. YIp204-PADH1-AFB2

   Plasmid

   #99531

   Purpose

   S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

   Depositor

   Helle Ulrich

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHes1-DsRed

   Plasmid

   #13767

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Hes1 promoter
   

   Tags

   DsRed2

   Expression

   Mammalian

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. p50 cFlag pcDNA3

   Plasmid

   #20018

   Depositor

   Stephen Smale

   Insert

   Nfkb1 (Nfkb1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Truncated at the p105 ligation site. It codes for…

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX393 Gluconacetobacter diazotrophicus Cas9

    Plasmid

    #68330

    Purpose

    Gluconacetobacter diazotrophicus Cas9

    Depositor

    Feng Zhang

    Insert

    GdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. eIF2a 3

    Plasmid

    #21809

    Depositor

    David Ron

    Insert

    eIF2a (EIF2S1 Human)
    

    Expression

    Mammalian

    Mutation

    S52D

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX383 Lactobacillus johnsonii Cas9

    Plasmid

    #68320

    Purpose

    Lactobacillus johnsonii Cas9

    Depositor

    Feng Zhang

    Insert

    LjCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX386 Lactobacillus farciminis Cas9

    Plasmid

    #68323

    Purpose

    Lactobacillus farciminis Cas9

    Depositor

    Feng Zhang

    Insert

    LfCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. wt HA-dynamin 1 pcDNA3.1

    Plasmid

    #34682

    Depositor

    Sandra Schmid

    Insert

    dynamin 1ba (DNM1L Human)
    

    Tags

    HA (variant)

    Expression

    Mammalian

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. p52 cFlag pcDNA3

    Plasmid

    #20019

    Depositor

    Stephen Smale

    Insert

    Nfkb2 (Nfkb2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Truncated at the p100 ligation site. It codes for…

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pXR003_puroR

    Plasmid

    #219819

    Purpose

    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.3_SARS2_XBB.1.5

    Plasmid

    #196585

    Purpose

    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (XBB.1.5)
    

    Expression

    Mammalian

    Mutation

    G252V, F486P on the basis of XBB

    Promoter

    CMV

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT3 p38

    Plasmid

    #12658

    Depositor

    John Kyriakis

    Insert

    p38alpha (MAPK14 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRL528

    Plasmid

    #58495

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al Methods Enzymol. 1988;167:747-54.

    Insert

    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Available Since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5 TBRI-His

    Plasmid

    #19161

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAIP4

    Plasmid

    #233686

    Purpose

    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.

    Depositor

    Christopher Bahl

    Insert

    Smt3 (SMT3 )
    

    Tags

    HisTag

    Promoter

    T7

    Available Since

    April 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. mCerulean C1

    Plasmid

    #27796

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Insert

    mCerulean C1
    

    Expression

    Mammalian

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pT7TS-rich

    Plasmid

    #99050

    Purpose

    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.

    Depositor

    Richard Bélanger

    Type

    Empty backbone

    Use

    Unspecified; Xenopus oocyte system

    Promoter

    pT7

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCI_FLAG-GFP(Y39TAG)-6His

    Plasmid

    #223507

    Purpose

    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

    Depositor

    Edward Lemke

    Insert

    GFP(Y39TAG)
    

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Y39TAG

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. FUGW-VGLUT1-pHluorin

    Plasmid

    #207470

    Purpose

    Lentiviral expression of VGLUT1-pHluorin

    Depositor

    Robert Edwards

    Insert

    VGLUT1 (Slc17a7 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAKlux2.1

    Plasmid

    #14081

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Bacterial luciferase
    

    Expression

    Bacterial

    Available Since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLenti6.2_mCherry_roGFP2_Orp1

    Plasmid

    #155043

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAcGP67A - murine TfR

    Plasmid

    #12392

    Depositor

    Pamela Bjorkman

    Insert

    transferrin receptor (Tfrc Mouse)
    

    Tags

    6X-His tag, Factor Xa cleavage site, and leader p…

    Expression

    Insect

    Mutation

    See comments section

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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29. QUE37C/pLBS.CAG

    Plasmid

    #129340

    Purpose

    ATP measurement, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Lentiviral

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMV4-HA

    Plasmid

    #27553

    Depositor

    Shao-Cong Sun

    Article

    Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

    Type

    Empty backbone

    Tags

    3x HA

    Expression

    Mammalian

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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