We narrowed to 11,283 results for: Vars;

1. pFLAG W96A-GLTP

   Plasmid

   #170741

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

   Insert

   GLTP (GLTP Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   W96A

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-PP-Spastin(87-616)DeltaExon4

   Plasmid

   #128794

   Purpose

   Bacteiral expression of Spastin M87 varient without exon 4

   Depositor

   Chris Hill

   Article

   Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

   Insert

   Spastin (SPAST Human)
   

   Use

   `

   Tags

   GST

   Expression

   Bacterial

   Mutation

   residues 87-616 without exon 4.

   Promoter

   T7

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-D2s-L-V2

   Plasmid

   #19968

   Depositor

   Jonathan Javitch

   Article

   Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

   Insert

   D2 short variant-C terminal Venus (DRD2 Human, Aequorea Victoria)
   

   Tags

   C terminal Venus and Flag

   Expression

   Mammalian

   Available Since

   Jan. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

4. MSP2372

   Plasmid

   #73196

   Purpose

   Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, and Q926A in SpCas9

   Promoter

   CMV

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TALEN-CCR5R18-CtermQ7

   Plasmid

   #51443

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) Q7 C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Mutation

   K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSCV Puro SCRIB P305L

   Plasmid

   #88887

   Purpose

   SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

   Depositor

   Senthil Muthuswamy

   Article

   Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

   Insert

   SCRIB (SCRIB Human)
   

   Use

   Retroviral

   Mutation

   Proline to Leucine mutation @ 305th amino acid

   Promoter

   MSCV promoter

   Available Since

   March 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RGS-6xHis-mRFP-pcDNA3.1-

   Plasmid

   #52516

   Purpose

   expresses mRFP in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   mRFP
   

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   engineered variant of tetrameric red fluorescent …

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2

   Plasmid

   #214814

   Purpose

   A single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATM

   Depositor

   Yannick Doyon

   Insert

   St1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

   Plasmid

   #101205

   Purpose

   Bacterial expression plasmid for SpCas9 REC3 domain

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K506–Q712
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K506–Q712

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFLAG CPTP

    Plasmid

    #170742

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

    Insert

    CPTP (CPTP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

    Plasmid

    #114101

    Purpose

    fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

    Insert

    Gaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGP-CMV-GCaMP3-44

    Plasmid

    #91780

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP3-44
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSET a sfGCaMP6s-T78H

    Plasmid

    #100023

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfGCaMP6s-T78H
    

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCI syn iGluf

    Plasmid

    #106121

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25D variant
    

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Glu 25 to Asp

    Promoter

    synapsin

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. alpha1Ddelta-G12-FRB

    Plasmid

    #87454

    Purpose

    rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    a1D short variants (Cacna1d Rat)
    

    Tags

    FRB

    Expression

    Mammalian

    Mutation

    delete KYPAKNTTIALQMLERML

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET51b-His-TEV-SNAP-tag_cx

    Plasmid

    #167273

    Purpose

    Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    P179R

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTH743-CEN-RLuc/slowstaCFLuc

    Plasmid

    #38223

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCELF PAI-RBP v3

    Plasmid

    #45507

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP v3 (SERBP1 Human, Rat)
    

    Tags

    GST tag

    Expression

    Mammalian

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCELF PAI-RBP v1

    Plasmid

    #45506

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP v1 (SERBP1 Human, Rat)
    

    Tags

    GST tag

    Expression

    Mammalian

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCELF PAI-RBP v2

    Plasmid

    #45505

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP v2 (SERBP1 Human, Rat)
    

    Tags

    GST tag

    Expression

    Mammalian

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pETDuet-solo-NHis6-NocO-D282A

    Plasmid

    #240407

    Purpose

    expresses N-His tagged NocO D282A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Asp…

    Promoter

    T7

    Available Since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Integrin β6β1-EGFP

    Plasmid

    #242829

    Purpose

    Encodes human integrin β6 chimera containing integrin β1 cytoplasmic domain and C-terminal EGFP tag

    Depositor

    Cheng-han Yu

    Article

    Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

    Insert

    Integrin subunit beta 6, transcript variant 1 (ITGB6 Human)
    

    Expression

    Mammalian

    Mutation

    Integrin β6 chimera containing the cytoplasmic ta…

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJRoC30-LacAnc100

    Plasmid

    #234653

    Purpose

    Expresses LacAnc100 variant in yeast

    Depositor

    Alice Ting, Miguel Alcalde Galeote

    Article

    Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

    Insert

    LacAnc100
    

    Tags

    HRV 3C protease site-GSG linker-8xHis tag

    Expression

    Yeast

    Promoter

    Gal1p

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. H-IRF7

    Plasmid

    #169785

    Purpose

    Express IRF7 in bacteria

    Depositor

    Sung-Liang Yu

    Article

    Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

    Insert

    interferon regulatory factor 7 (IRF7), transcript variant a (IRF7 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pETDuet-solo-NHis6-NocO-H286A

    Plasmid

    #240409

    Purpose

    expresses N-His tagged NocO H286A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, His…

    Promoter

    T7

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pETDuet-solo-NHis6-NocO-H132A

    Plasmid

    #240404

    Purpose

    expresses N-His tagged NocO H132A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, His…

    Promoter

    T7

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETDuet-solo-NHis6-NocO-E129A

    Plasmid

    #240403

    Purpose

    expresses N-His tagged NocO E129A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETDuet-solo-NHis6-NocO-Q128A

    Plasmid

    #240402

    Purpose

    expresses N-His tagged NocO Q128A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pETDuet-solo-NHis6-NocO-E252A

    Plasmid

    #240405

    Purpose

    expresses N-His tagged NocO E252A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pETDuet-solo-NHis6-NocO-E283A

    Plasmid

    #240408

    Purpose

    expresses N-His tagged NocO E283A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge