We narrowed to 8,865 results for: APP

Showing: 6841 - 6860 of 8865 results

Results per page:

Sort results by:

pDONR223-ZNF655

Plasmid

#88578

Purpose

Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF655 (ZNF655 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_PGK-hACE2

Plasmid

#161612

Purpose

Expression of human ACE2 in mammalian cells

Depositor

Brad Rosenberg

Article

Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKK-mCherry-TEV

Plasmid

#105779

Purpose

Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mCherry-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD-4D

Plasmid

#178672

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues removed (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD-4D (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

four Asp residues removed

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 S_nostop

Plasmid

#152988

Purpose

Gateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221-ZNF736

Plasmid

#101602

Purpose

Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF736 (ZNF736 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-DIO-FLPo-Myc

Plasmid

#124641

Purpose

AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

FLPo

Use

AAV and Cre/Lox

Tags

myc tag

Expression

Mammalian

Promoter

EF1a

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_KLF4_P2A_Hygro_Barcode

Plasmid

#120455

Purpose

Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-Notch1_E6-pBIOCAM5

Plasmid

#39344

Depositor

John McCafferty

Article

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Mouse, Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT1:Cas9-GFP, U6:sgUPRT

Plasmid

#122853

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgUPRT

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKK-BI16-TEV-mCherry

Plasmid

#105800

Purpose

Concomitant expression of two proteins. One protein is expressed with mCherry fusion tag (at the C-terminus), cleavable by TEV protease; second tag introduced by user.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mCherry

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZLblasti-HA-ERK2 GOF

Plasmid

#53174

Purpose

Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Mustard Weed, Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S/D321N

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3-AUP1-FLAG-DCUE

Plasmid

#185339

Purpose

Assessing domain requirements of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 293-337 corresponding to the CUE d…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLC-BFP-FADD

Plasmid

#75166

Purpose

LentiCRISPR-BFP with sgRNA targeting human FADD

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

FADD sgRNA (FADD Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF574

Plasmid

#88644

Purpose

Donor Vector containing ZNF574 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF574 (ZNF574 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TVBB C-term-mTurquoise2-Hygro

Plasmid

#169225

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Hygro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAuroraA GFP-AURKA-GFP

Plasmid

#157765

Purpose

Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter

Depositor

Marc Tramier

Article

Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Mammalian

Mutation

promoter CMV replaced by AurkA promoter

Promoter

AURKA minimal

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKK-mRuby3-TEV

Plasmid

#105780

Purpose

Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mRuby3-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER-21-LCOR

Plasmid

#97038

Purpose

Expresses LCOR in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor (LCOR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FCM101

Plasmid

#194298

Purpose

E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6

Depositor

Shohei Koide

Article

Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:

Insert

FCM101

Tags

His tag, FLAG tag

Expression

Bacterial

Promoter

T7

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge