We narrowed to 11,684 results for: cel.2

1. HA-HIF2alpha-P405A/P531A-pBabe-puro

   Plasmid

   #19006

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: P405A and P531A

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-Flag-hSox2

   Plasmid

   #20073

   Depositor

   Juan Belmonte

   Article

   Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

   Insert

   hSox2 (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-PGK-KRAS4A(G12V)

   Plasmid

   #35634

   Depositor

   Daniel Haber

   Article

   Singh et al Cell. 2012 Feb 17;148(4):639-50.

   Insert

   K-Ras4A(G12V) (KRAS Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Glycine 12 to Valine

   Promoter

   PGK

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

4. eSpCas9-EF-5Nanog_2

   Plasmid

   #174871

   Purpose

   CRISPR vector for generating Nanog STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Nanog (Nanog Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. FR_HNF1B

   Plasmid

   #31101

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. ATG-1929

   Plasmid

   #108712

   Purpose

   CBR2opt in pF4Ag expression vector with T7 and CMV promoter

   Depositor

   Keith Wood

   Article

   Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

   Insert

   CBR2opt
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promega used directed evolution to create CBR (Cl…

   Promoter

   T7, CMV

   Available since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC149-pCR8-dCas9VP160

   Plasmid

   #48221

   Purpose

   dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP160

   Expression

   Mutation

   D10A;H840A nuclease-deficient

   Promoter

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTBRII / CEP-Zeo / Hygro

   Plasmid

   #16622

   Depositor

   Bert Vogelstein

   Article

   Zhou et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Insert

   TGF beta type II receptor (TGFBR2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pDECKO_mCherry_non-targeting

   Plasmid

   #140109

   Purpose

   CRISPR-Cas9 library validation (negative control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. tetO-MESP1

    Plasmid

    #170694

    Purpose

    doxycycline-inducible overexpression of MESP1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    MESP1 (MESP1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid

    #160742

    Purpose

    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330A-Yap1-E1-#1

    Plasmid

    #171517

    Purpose

    deletion of a genomic locus in Yap1 gene

    Depositor

    Hans Schöler

    Article

    Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

    Insert

    Yap1 (Yap1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330A-Yap1-E2-#1

    Plasmid

    #171518

    Purpose

    deletion of a genomic locus in Yap1 gene

    Depositor

    Hans Schöler

    Article

    Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

    Insert

    Yap1 (Yap1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. JPF0535a

    Plasmid

    #124042

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDECKO_mCherry_CEACAM1

    Plasmid

    #140102

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDECKO_mCherry_Rat_cebp-alfa

    Plasmid

    #140098

    Purpose

    CRISPR-Cas9 library validation (positive control)

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDECKO_mCherry_SPI1

    Plasmid

    #140099

    Purpose

    CRISPR-Cas9 library validation (positive control)

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC_C_HSP7C

    Plasmid

    #111667

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    HSP7C (HSPA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. 200 bp Axin2 mutant

    Plasmid

    #25704

    Depositor

    Eric Fearon

    Article

    Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

    Insert

    Axin 2 promoter (AXIN2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    TCF DNA binding site at bp -108 to -102 mutated …

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

20. p.UTA.2.0 miR-27a-3p 3 perfect copies.

    Plasmid

    #82439

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Use

    Tags

    Expression

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. FR_HNF1B_A263insGG

    Plasmid

    #31103

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    mutation A263insGG

    Promoter

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. D998-EGFR-ET1

    Plasmid

    #44186

    Depositor

    Timothy Springer

    Article

    Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

    Insert

    D998-EGFR-ET1 (EGFR Human)
    

    Use

    Tags

    3C cleavge site, protein C peptide tag, his6, SBP…

    Expression

    Mammalian

    Mutation

    EGFR amino acids 1−998

    Promoter

    CVM

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

23. ARB365

    Plasmid

    #124048

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FR_HNF1B_P328L329del

    Plasmid

    #31102

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    mutation P328L329delCCTCT

    Promoter

    Available since

    Oct. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGFP AH2 tensin

    Plasmid

    #50514

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Article

    Pankov et al J Cell Biol. 2000 Mar 6;148(5):1075-90.

    Insert

    Tensin (TNS1 Chicken)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    AA659--761, including actin homology 2 domain of …

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pAc5.1B-EGFP-DmMextli

    Plasmid

    #79262

    Purpose

    Expresses EGFP-tagged DmMextli in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

    Insert

    DmMextli (mxt Fly, NM_164598)
    

    Use

    Schneider 2 cell expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. PCDNA3.1:mTRESK

    Plasmid

    #224771

    Purpose

    In vitro transcription for Oocyte expression of mTRESK

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 18 (Kcnk18 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1:mTRESK(S116C)

    Plasmid

    #224772

    Purpose

    In vitro transcription for Oocyte expression of mTRESK(S116C)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 18 (Kcnk18 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S116C

    Promoter

    T7

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEMHE:mTREK-1(S131C)

    Plasmid

    #224744

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(S131C)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEMHE:mTREK-1(S131A)

    Plasmid

    #224745

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(S131A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131A

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge