We narrowed to 12,476 results for: pcdna

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL

Plasmid

#187079

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in endoplasmic reticulum

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194381

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1

Plasmid

#187586

Purpose

Express FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

EWSR1 (EWSR1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7

Plasmid

#74932

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

Plasmid

#100279

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Mutated catalytic domain (1025aa-End) (please see…

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

Plasmid

#187578

Purpose

Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

WRAP53 (WRAP53 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N

Plasmid

#193999

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181-D174N

Depositor

Christina Woo

Article

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181-D174N

Tags

myc

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Plk4(Sak) D154A (Nigg HR26)

Plasmid

#41164

Depositor

Erich Nigg

Article

Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

Insert

Plk4 D154A (PLK4 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

D154A

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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3341 pcDNA3 Bcl-2 T69A S70A S87A

Plasmid

#13340

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Mutation

changed Threonine 69 to Alanine; changed Serine 7…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pcDNAz-mBACEflag (C474A/C478A/C482A/C485A)

Plasmid

#26736

Depositor

Gopal Thinakaran

Article

Vetrivel et al J Biol Chem. 2009 Feb 6. 284(6):3793-803

Insert

BACE (Bace1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Cys 474, 478, 482, and 485 mutated to Ala

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

Plasmid

#168192

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-LaG6-(EAAAK)4-OGT(4)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)

Plasmid

#204696

Purpose

This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT(deltaMIN) (BRD4, NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of the amino acids 355-505 of NUT

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

Plasmid

#204695

Purpose

This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT(MIN) (BRD4, NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of majority of NUT, retaining only amino…

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

Plasmid

#164845

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag

Depositor

Erik Procko

Article

Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

Insert

Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)

Tags

8his tag and HA leader sequence

Expression

Mammalian

Mutation

Codon-optimized for human cell expression. Has N5…

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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(318) pcDNA3.1-myc-OGA(1-400)D174N

Plasmid

#168096

Purpose

Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

myc-OGA(1-400, D174N)

Tags

myc

Expression

Mammalian

Mutation

D174N catalytically inactive OGA

Promoter

T7/CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-S28 histone 3 phosphorylation reporter-NLS

Plasmid

#32787

Depositor

Alice Ting

Article

Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

Insert

CFP-14-3-3tau-H3 peptide-YFP

Tags

CFP and YFP

Expression

Mammalian

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

Plasmid

#164013

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R464E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

Plasmid

#125796

Purpose

for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CD40L (lose of function mutant)-mgSrtA

Expression

Mammalian

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3- myc NES-mCE(K294A) NLS

Plasmid

#82467

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NLS deleted from mouse Capping Enzyme (Rngtt Mouse)

Expression

Mammalian

Mutation

deletion of KRKY from 573-657

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-1-101-FLAG basic mutant

Plasmid

#35985

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV1, 101 aa; R to A mutations at aa 56,…

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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